Bayer HealthCare ADVIA 60, Руководство оператора

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3. SPECIFICATIONS
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HGB (Hemoglobin):
Turbidity of the blood sample - Due to any number of physiologic and/or therapeutic factors
may produce falsely elevated HGB results. To obtain accurate hemoglobin results when increased
turbidity of the blood sample occurs, determine the cause of the turbidity and follow the appropriate
method below :
1. Elevated WBC : An extremely elevated WBC will cause excessive light scatter. In these
cases use reference (manual) methods.The diluted sample should be centrifuged, and the
supernatant fluid measured on a spectrophotometer.
2. Elevated lipids : Elevated lipids in the blood sample will give the plasma a "milky" appearance.
This condition can occur with hyperlipidemia, hyperproteinemia (as in gammapathies) and
hyperbilirubinemia. Accurate hemoglobin determinations can be achieved by using reference
(manual) methods and a plasma blank.
Increased turbidity may also be seen in case where the red blood cells are resistant to lysing.
This condition will cause a falsely elevated Hgb result, but may be detected by observing the
abnormal MCH, MCHC values, and the increased baseline on the leading edge of the WBC
histogram. Erroneous hemoglobin results will cause the results of the MCH and MCHC to be
erroneous as well.
Fetal bloods - The mixing of fetal and maternal bloods may produce a falsely elevated HGB
value.
HCT (Hematocrit):
Red blood cells agglutination - May produce erroneous HCT and MCV values. Red blood cells
agglutination may be detected by observing the abnormal MCH and MCHC values, as well as by
examination of the stained blood film In such cases, manual methods may be required to obtain
an accurate HCT value.
MCV (Mean Corpuscular Volume):
Red blood cell agglutination - May produce an erroneous MCV value. Red blood cell agglutina-
tion may be detected by observing the abnormal MCH and MCHC values, as well as by examination
of the stained blood film. In such cases, manual methods may be required to obtain an accurate
MCV value.
Excessive numbers of large platelets and/or the presence of an excessively high WBC
count
- May interfere with the accurate determination of the MCV value. In such cases, careful
examination of the stained blood film may reveal the error.
MCH (Mean Corpuscular Hemoglobin):
The MCH is a function of th HGB value and the RBC count. The limitations listed for the HGB and
RBC will have an effect on the MCH and may cause erroneous values.
MCHC (Mean Corpuscular Hemoglobin Concentration):
The MCHC is a function of the HGB and HCT values. The limitations listed for the HGB and HCT
will have an effect on the MCHC and may cause erroneous values.