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OPERATION
Carl Zeiss
Illumination and contrasting techniques
Axiovert 200
3-38
B 40-080 e 03/01
3.3.4
Setting of VAREL contrast in transmitted light
3.3.4.1
General principle
VAREL contrast provides a relief-like image of objects and can be used as an alternative to phase
contrast. VAREL contrast can also be used on curved surfaces, e.g. 96-well microtiter plates, for which
no contrast can be achieved with the phase contrast technique (no flush ring positioning!).
3.3.4.2
Configuration of the Axiovert 200 (manual) and Axiovert 200 M
−
Condenser with turret equipped with VAREL stops.
−
Objectives which are suitable for VAREL contrast, e.g. A-Plan Ph1Var1 or LD A-Plan Ph2Var2.
3.3.4.3
Setting of VAREL on the Axiovert
200 (manual) and Axiovert 200 M
•
Open the aperture diaphragm (3-21/
1
)
completely.
•
Swing VAREL stop on the turret of the
condenser into the beam path (
Var
position).
•
Swing in the required VAREL objective via the
nosepiece.
•
Use the setting screw (3-21/
2
) to move the
Varel diaphragm opening until optimum VAREL
contrast is achieved (relief-like impression).
☞
Microtiter plates:
Select the opposite VAREL ring of the diaphragm for illumination at the edge of the cavity; in
the center of the cavity, the right or left VAREL ring can be used.
Fig. 3-21
Setting of VAREL contrast
Summary of Contents for Axiovert 200
Page 1: ...Operating Manual Axiovert 200 Axiovert 200 M Inverted Microscopes...
Page 30: ...INSTRUMENT DESCRIPTION Carl Zeiss Technical Data Axiovert 200 1 16 B 40 080 e 03 01...
Page 34: ...START UP Carl Zeiss List of illustrations Axiovert 200 2 4 B 40 080 e 03 01...
Page 114: ...ANNEX Carl Zeiss Overview Axiovert 200 A 2 B 40 080 e 03 01...
Page 120: ...ANNEX Carl Zeiss List of key words Axiovert 200 A 8 B 40 080 e 03 01...