YL Instruments YL9100 HPLC, User Manual

Page: 27 / 188

YL9110 Quaternary Pump
Chapter 4. Operation
There are four LEDs in front of YL9110 quaternary pump.
Y
L9110
POWER LED turns ON if main power turns on
CONNECTED LED turns ON if communication is connected,
LED blinks during connection
READY/RUN LED turns ON before analysis, LED blinks during analysis
ERROR LED turns ON if there is error
4-1. Before Start
When using pump for the first time, initialize it through the following process in order to clean flow path
and condition high pressure seal. This process is necessary in case instrument is installed newly or is not
used for long time.
1) Prepare iso-propanol of HPLC grade.
2) Remove residual air bubble within instrument by turning prime/purge valve in counter clockwise
and loading iso-propanol of at least 50ml by Prime Start button.
3) Separate pump outlet tubing.
4) Press the sucked iso-propanol into syringe with prime/purge valve and discharge more than 5ml
to outlet of in-line filter.
5) Operate pump with instrument outlet open for 2-3 hours at 0.2ml/min flow rate and for 1 hour at
1.0ml/min flow rate using iso-propanol.
6) Perform process of 2) ~ 5) using solvent which will be used as a mobile phase.
7) Remove inside residual iso-propanol by operating it at 1ml/min flow rate for 30 minutes with
instrument outlet open.
8) Form flow path by connecting injector, column, and detector tubing mutually.
15