YL Instruments YL9100 HPLC, User Manual

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YL9160 PDA Detector
5) Appropriate solvents, reagents, etc
6) Nuts, ferrules, appropriate to the column end-fittings being used
7) Wrenches appropriate to column end-fittings
8) Connecting tubing and union (if column cannot be connected directly to the cell).
2-2. Connection of power
Confirm supplying voltage, electric power, socket, and type of connector are correct. Before connect
power cable on the pump, please turn off the power switch of YL9160 PDA Detector.
Warning ! : The electric power should be matched with description on the rear side of instrument. If not,
the instrument can be damaged or it can be a reason of fire.
Caution ! : Confirm the ground is correct. Water pipe is not recommended in case of non-metal pipe. Gas
pipe should not use because of safety. If the ground is not correct, the electric shock can be happened.
2-3. Connection of a column
The length of tubing between the inlet of flow cell assembly and outlet of column should be connected
as short as possible. It is the ideal that you connect the tubing directly between these two ends. If this is
not possible, you should use a minimum length of narrow bore (0.010 inch I.D.) connecting tubing and a
zero dead volume union. Because different columns use different fittings, the detector is supplied with a
bare tube end to allow connection to any column accepting 1/16 inch O.D. tubing. You should use nuts
and ferrules suitable to your column.
Y
L9160
NOTE: Tubing size and position is different for the adjustable path length preparative flow cells, high
pressure narrow-bore flow cell, off column capillary flow cell, and on column capillary flow cell.
See their owner's manuals for details.
Connect the cell outlet (the upper of the two tubes which protrude from the rear wall of the cell
compartment) to a line leading to an appropriate waste reservoir. If bubble formation in the detector cell
causes problems, you may wish to connect the cell outlet to a restrictor or back pressure device providing
20-60 psi back-pressure.
NOTE: Before connecting any new tube or column to the detector, flow several mL of clean solvent
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