YL Instruments YL9100 HPLC User Manual Download Page 183

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YL9170 RI Detector

Make sure that only 2/3 of the slit are illuminated by the light source. This is illustrated in the following

figure.

[Slit aperture]

NOTE

The projected picture at the light sensor should be a sharp one. The full height of the photodiodes has to

be illuminated by the light beam. This is shown in the following figure.

L9170

[Photodiode]

Summary of Contents for YL9100 HPLC

Page 1: ......

Page 2: ...YL9100 HPLC SYSTEM YL9101 VACUUM DEGASSER USER MANUAL...

Page 3: ...3 Connection of tubing 3 2 3 1 Connecting of mobile phase solvent 3 2 3 2 Connecting of Solvent delivery pump 3 Chapter 3 Operation 4 3 1 Degassing of the solvent 4 3 2 Operating of solvent delivery...

Page 4: ...lent environment for HPLC analysis YL9101 Vacuum Degasser consists of four channels for degassing and each channel removes efficiently air and oxygen in the solvent Also solvents which were degassed b...

Page 5: ...ere not receive electromagnetic induction from large transformer high frequency heater UPS etc 9 Within 2500 m above sea level storage within 4600m Please check the following before you install the sy...

Page 6: ...st plug up the solvent port which is not used Open a dust cover in the front you will see 8 ports 4 ports left are for input and right 4 ports are for output Use a plug screw supplied to plug up un us...

Page 7: ...emove the bubble open the prime purge valve and pull out air bubble by syringe slowly until the bubbles are removed After remove close the valve for normal use of pump 3 3 Replacing solvents When nece...

Page 8: ...tion of input port to output port Do not connect it by serial way 4 Can not use YL9101 Vacuum Degasser for output of solvent delivery pump 4 3 Troubleshooting 1 Error and malfunctioning Errors of YL91...

Page 9: ...YL9100 HPLC SYSTEM YL9110 QUATERNARY SOLVENT DELIVERY PUMP USER MANUAL...

Page 10: ...7 Chapter 3 Installation 8 3 1 Inspection and site preparation 8 3 2 Connection of power 9 3 3 Connection of tubing 9 3 4 Connection of remote cable 12 3 5 Connection of communication cable 14 Chapter...

Page 11: ...e 23 5 1 Caution 23 5 2 Replacement of high pressure seal 23 5 3 Replacement of plunger 25 5 4 Replacement of check valve cartridge 26 5 5 Replacement of low pressure seal 26 5 6 Cleaning of flow path...

Page 12: ...n YL9110 quaternary pump and YL9100 HPLC system 11 Fig 7 Tubing connection of rinse port 11 Fig 8 Connection of remote cable between YL9110 pump and injector 12 Fig 9 Pin configuration of each termina...

Page 13: ...the life time of pump head Using UHMWPE seal it provides extended life time of high pressure seal even though use buffer solution as a mobile phase On the outlet of pump there is a in line filter to...

Page 14: ...Composition Accuracy 0 5 12 Semi Auto prime purge 13 Communications LAN 14 Safety maintenance Leak detection Diagnostics Error detection 15 Dimensions 385 X 160 X 565 mm width X height X depth 16 Line...

Page 15: ...tlet check valve Fig 1 Flow diagram of YL9110 pump Solvent Filter The solvent filter is used for protecting the system from the particles in solvent This filter removes particles from solvent to prolo...

Page 16: ...button on the software to operate the micro pump Prime micro pump Using this pump the pump circulates rinse solvent into the pump head Solenoid valve It is mixing valve for low pressure gradient YL91...

Page 17: ...ump is controlled in real time so that occurrence of pulse proportional to pressure may be depressed Control method of the Pump uses supervision of pressure and control of location simultaneously so i...

Page 18: ...rol YL9110 quaternary pump controls open close signal of solenoid valve to perform low pressure gradient elution For accuracy and precision of solvent mixing YL9110 quaternary pump controls valve open...

Page 19: ...YL9110 quaternary pump has a exponential curve program but use gradient elution by linear curve when it operates with YLClarity software Fig 4 Gradient curve 2 6 Rinse port When using buffer solution...

Page 20: ...and all other sides of pump with buffer material in order to make pump endure shock or vibration during transportation Standard configuration of YL9110 quaternary pump 1 Main body of instrument 2 Powe...

Page 21: ...and type of connector are correct Before connect power cable on the pump please turn off the power switch of YL9110 quaternary pump Warning The electric power should be matched with description on th...

Page 22: ...r should be used plastic tubing cutter or shaving cutter should be used for teflon and similar material of tubing and the surface should be clean and have no crumbling Fig 5 Fitting for 1 16 OD tubing...

Page 23: ...rt tubing connects on the each pump head using 1 4 28 fitting as same as Fig 7 and fill the 20 50 Methanol The rinse pump inside of YL9110 quaternary pump circulates the rinse solvent into the pump he...

Page 24: ...cted on the Remote In terminal on the rear side of YL9110 quaternary pump to collect data at the moment of injection Fig 8 Connection of remote cable between YL9110 pump and injector Notice Please do...

Page 25: ...series modules connect directly and confirm the configuration with the other modules YL9110 Fig 10 Diagram of Remote and Marker input Fig 11 Diagram of Remote and Marker output YL9110 quaternary pump...

Page 26: ...e event program output READY IN To change error state and stop operation if there is a input READY OUT When YL9110 quaternary pump is not ready state because of running status output error signal is i...

Page 27: ...long time 1 Prepare iso propanol of HPLC grade 2 Remove residual air bubble within instrument by turning prime purge valve in counter clockwise and loading iso propanol of at least 50ml by Prime Start...

Page 28: ...during actual analysis Solvent gas removal and filtration are necessary because they have great effect on result of analysis and maintenance of instrument 4 3 1 Degassing Solvent gas removal is perfor...

Page 29: ...ore use 4 3 3 Solvent effect on the instrument All parts of the Gradient Pump contacting with mobile phase is manufactured from 316 stainless steel ruby sapphire zirconium or fluorine carbon polymer M...

Page 30: ...ress out iso propanol sucked into syringe in prime purge valve and discharge at least 5ml into outlet of in line filter 5 Separate mobile phase filter assembly and block discharge hole and suction hol...

Page 31: ...click arrow button to move this on the right window Click red arrow button on the right bottom side and select the pressure unit Double click YL911x pump on the right window and check IP address of p...

Page 32: ...Resume Idle If you click this button the pump goes to idle state Prime Start If you click this button the pump runs at high speed with prime pump to fill the solvent into the lines This function works...

Page 33: ...f the pump stops Diagnosis It is displayed during self test 4 4 3 Method Setup In this window edit time program table and setup the pump status during idle state Click Options button to setup Max and...

Page 34: ...is opposite when you use with the other device change it on the External In Out Set 4 4 4 Error message If there are errors on the pump caused by pressure limit control value range and leak the pump s...

Page 35: ...hloride Liquid Chlorine Methylene Chloride Tetrahydrofuran Teflon PTFE Dimethyl Formamide Diethylamine SS316 Phosphoric Acid Conc Rm Temp 3 Do not install instrument where corrosive gas is generated o...

Page 36: ...seal with seal insertion removal tool and insert new high pressure seal prepared at that place using seal insertion removal tool in the same manner Direction of seal should be such that the direction...

Page 37: ...wear is not well observed visually so care should be taken when observing it 1 Loosen tubing of inlet check valve and outlet check valve of pump head 2 Press pump head to main body of instrument by ha...

Page 38: ...idge to remove the nitric acid used for cleaning 5 Assemble loosened check valve in the reverse order 5 5 Replacement of low pressure seal Wearing of the low pressure seal is caused when pump has been...

Page 39: ...head into parts in the same manner as seal change process of 5 2 wash it with ultrasonic cleaner and assemble each part again At this time the high pressure seal is damaged so replace it with new one...

Page 40: ...pe is slippery separate it with tubing held avoiding slippage using 1000 sand paper 2 In case of performing ultrasonic wave cleaning wash head part by ultrasonic cleaner with 10 nitric acid solution f...

Page 41: ...e pump can be damaged by the crystallization of salt growth of microorganism contamination To maintain the pump properly run the pump using iso propanol for 30 minutes and the plug the outlet of pump...

Page 42: ...p head Air separation status of solvent is bad Check valve is not good Liquid containing oil flows into head part High pressure seal was worn Perform prime purge again Take measures so that air separa...

Page 43: ...al Pump is not operated after input of power Power voltage is unstable or low Strong induction voltage is generated in the surroundings Use stable proper DC power source Close induction power source i...

Page 44: ...YL9100 HPLC SYSTEM YL9111 BINARY PUMP USER MANUAL...

Page 45: ...Inspection and site preparation 8 3 2 Connection of power 9 3 3 Connection of tubing 9 3 4 Connection of remote cable 12 3 5 Connection of communication cable 14 Chapter 4 Operation 15 4 1 Before Star...

Page 46: ...11 5 2 Replacement of high pressure seal 23 5 3 Replacement of plunger 25 5 4 Replacement of check valve cartridge 26 5 5 Replacement of low pressure seal 26 5 6 Cleaning of flow path within pump 26 5...

Page 47: ...L9111 Binary pump and YL9100 HPLC system 11 Fig 7 Tubing connection of rinse port 11 Fig 8 Connection of remote cable between YL9111 pump and injector 12 Fig 9 Pin configuration of each terminal 12 Fi...

Page 48: ...life time of pump head Using UHMWPE seal it provides extended life time of high pressure seal even though use buffer solution as a mobile phase On the outlet of pump there is a in line filter to preve...

Page 49: ...Accuracy 0 5 10 Auto prime purge 11 Communications LAN 12 Safety maintenance Leak detection Diagnostics Error detection 13 Dimensions 385 X 160 X 565mm width X height X depth 14 Line Voltage 100 240V...

Page 50: ...k valve YL9111 Fig 1 Flow diagram of YL9111 pump Solvent Filter The solvent filter is used for protecting the system from the particles in solvent This filter removes particles from solvent to prolong...

Page 51: ...n the prime purge valve and suck the solvent using syringe or click Prime Start button on the software to operate the micro pump Prime micro pump Using this pump the pump circulates rinse solvent into...

Page 52: ...is controlled in real time so that occurrence of pulse proportional to pressure may be depressed Control method of the Pump uses supervision of pressure and control of location simultaneously so it ha...

Page 53: ...ow rate Fab Solvent volume V of solvent A vs Concentration of solvent B C 100 x 1 Fab Fb V x t Fb Fab Fb The solvent ratio difference between present row and next row increases or decreases as a expon...

Page 54: ...to cause shortening of seal life which has bad effect on pump Rinse port enables to insert proper solvent in back side of high pressure seal to prevent salts from being deposited and activated Mixed...

Page 55: ...and all other sides of pump with buffer material in order to make pump endure shock or vibration during transportation Standard configuration of YL9111 Binary pump 1 Main body of instrument 2 Power co...

Page 56: ...et and type of connector are correct Before connect power cable on the pump please turn off the power switch of YL9111 Binary pump Warning The electric power should be matched with description on the...

Page 57: ...should be used plastic tubing cutter or shaving cutter should be used for teflon and similar material of tubing and the surface should be clean and have no crumbling Fig 5 Fitting for 1 16 OD tubing I...

Page 58: ...tubing connects on the each pump head using 1 4 28 fitting as same as Fig 7 and fill the 20 50 Methanol The rinse pump inside of YL9111 Binary pump circulates the rinse solvent into the pump head eve...

Page 59: ...cted on the Remote In terminal on the rear side of YL9111 Binary pump to collect data at the moment of injection Fig 8 Connection of remote cable between YL9111 pump and injector Notice Please do not...

Page 60: ...series modules connect directly and confirm the configuration with the other modules YL9111 Fig 10 Diagram of Remote and Marker input Fig 11 Diagram of Remote and Marker output YL9111 Binary pump pro...

Page 61: ...ime event program output READY IN To change error state and stop operation if there is a input READY OUT When YL9111 Binary pump is not ready state because of running status output error signal is ind...

Page 62: ...time 1 Prepare iso propanol of HPLC grade 2 Remove residual air bubble within instrument by turning prime purge valve in counter clockwise and loading iso propanol of at least 50ml by Prime Start but...

Page 63: ...ring actual analysis Solvent gas removal and filtration are necessary because they have great effect on result of analysis and maintenance of instrument 4 3 1 Degassing Solvent gas removal is performe...

Page 64: ...e use 4 3 3 Solvent effect on the instrument All parts of the Gradient Pump contacting with mobile phase is manufactured from 316 stainless steel ruby sapphire zirconium or fluorine carbon polymer Mos...

Page 65: ...ss out iso propanol sucked into syringe in prime purge valve and discharge at least 5ml into outlet of in line filter 5 Separate mobile phase filter assembly and block discharge hole and suction hole...

Page 66: ...click arrow button to move this on the right window Click red arrow button on the right bottom side and select the pressure unit Double click YL911x pump on the right window and check IP address of p...

Page 67: ...sume Idle If you click this button the pump goes to idle state Prime Start If you click this button the pump runs at high speed with prime pump to fill the solvent into the lines This function works o...

Page 68: ...ump stops Diagnosis It is displayed during self test 4 4 3 Method Setup In this window edit time program table and setup the pump status during idle state YL9111 Click Options button to setup Max and...

Page 69: ...opposite when you use with the other device change it on the External In Out Set 4 4 4 Error message If there are errors on the pump caused by pressure limit control value range and leak the pump sto...

Page 70: ...Teflon PTFE Dimethyl Formamide Diethylamine SS316 Phosphoric Acid Conc Rm Temp 3 Do not install instrument where corrosive gas is generated or where there is carpet on floor 4 Do not change flow rate...

Page 71: ...h pressure seal prepared at that place using seal insertion removal tool in the same manner Direction of seal should be such that the direction to see O ring is toward front of head Be careful not to...

Page 72: ...is not well observed visually so care should be taken when observing it 1 Loosen tubing of inlet check valve and outlet check valve of pump head 2 Press pump head to main body of instrument by hand a...

Page 73: ...ge to remove the nitric acid used for cleaning 5 Assemble loosened check valve in the reverse order 5 5 Replacement of low pressure seal Wearing of the low pressure seal is caused when pump has been u...

Page 74: ...head into parts in the same manner as seal change process of 5 2 wash it with ultrasonic cleaner and assemble each part again At this time the high pressure seal is damaged so replace it with new one...

Page 75: ...is slippery separate it with tubing held avoiding slippage using 1000 sand paper 2 In case of performing ultrasonic wave cleaning wash head part by ultrasonic cleaner with 10 nitric acid solution for...

Page 76: ...pump can be damaged by the crystallization of salt growth of microorganism contamination To maintain the pump properly run the pump using iso propanol for 30 minutes and the plug the outlet of pump an...

Page 77: ...head Air separation status of solvent is bad Check valve is not good Liquid containing oil flows into head part High pressure seal was worn Perform prime purge again Take measures so that air separati...

Page 78: ...ump is not operated after input of power Power voltage is unstable or low Strong induction voltage is generated in the surroundings Use stable proper DC power source YL9111 Close induction power sour...

Page 79: ...YL9100 HPLC SYSTEM YL9112 ISOCRATIC PUMP USER MANUAL...

Page 80: ...7 3 2 Connection of power 8 3 3 Connection of tubing 8 3 4 Connection of remote cable 11 3 5 Connection of communication cable 13 Chapter 4 Operation 15 4 1 Before Start 15 4 2 Mobile phase filter and...

Page 81: ...essure seal 23 5 3 Replacement of plunger 25 5 4 Replacement of check valve cartridge 26 5 5 Replacement of low pressure seal 26 5 6 Cleaning of flow path within pump 26 YL9112 5 7 Supply of lubricant...

Page 82: ...tem 10 Fig 7 Tubing connection of rinse port 10 YL9112 Fig 8 Connection of remote cable between YL9112 pump and injector 11 Fig 9 Pin configuration of each terminal 12 Fig 10 Diagram of Remote and Mar...

Page 83: ...the pump has a automatic rinsing function to prolong the life time of pump head Using UHMWPE seal it provides extended life time of high pressure seal even though use buffer solution as a mobile phase...

Page 84: ...5 10 Auto prime purge 11 Communications LAN 12 Safety maintenance Leak detection Diagnostics Error detection 13 Dimensions 385 X 160 X 565mm width X height X depth 14 Line Voltage 100 240VAC 10 autom...

Page 85: ...ilter The solvent filter is used for protecting the system from the particles in solvent This filter removes particles from solvent to prolong the life time of high pressure seal and prevent damage on...

Page 86: ...or click Prime Start button on the software to operate the micro pump Prime micro pump Using this pump the pump circulates rinse solvent into the pump head Mixer It is static mixer to improve mixing e...

Page 87: ...mp is controlled in real time so that occurrence of pulse proportional to pressure may be depressed Control method of the Pump uses supervision of pressure and control of location simultaneously so it...

Page 88: ...k side of high pressure seal and these deposits wear pump seal to cause shortening of seal life which has bad effect on pump Rinse port enables to insert proper solvent in back side of high pressure s...

Page 89: ...all other sides of pump with buffer material in order to make pump endure shock or vibration during transportation YL9112 Standard configuration of YL9112 isocratic pump 1 Main body of instrument 2 Po...

Page 90: ...d type of connector are correct Before connect power cable on the pump please turn off the power switch of YL9112 isocratic pump Warning The electric power should be matched with description on the re...

Page 91: ...hould be used plastic tubing cutter or shaving cutter should be used for teflon and similar material of tubing and the surface should be clean and have no crumbling YL9112 Fig 5 Fitting for 1 16 OD tu...

Page 92: ...ubing connects on the each pump head using 1 4 28 fitting as same as Fig 7 and fill the 20 50 Methanol The rinse pump inside of YL9112 isocratic pump circulates the rinse solvent into the pump head ev...

Page 93: ...utput external solenoid valve and marker input output The remote cable from the injector manual or autosampler has to be connected on the Remote In terminal on the rear side of YL9112 isocratic pump t...

Page 94: ...n and confirm with YL9112 isocratic pump terminal configuration Fig 9 Pin configuration of each terminal Fig 10 and Fig 11 are the diagram of remote and the other terminal input output In between YL91...

Page 95: ...zation of remote start with the other instrument MARK IN To control event program or operate additional operation MARK OUT To control time event program output READY IN To change error state and stop...

Page 96: ...YL9112 Isocratic Pump Fig 13 Connection of communication cable Notice The LAN HUB used for cable connection on the PC must use switching mode module 14...

Page 97: ...ong time 1 Prepare iso propanol of HPLC grade 2 Remove residual air bubble within instrument by turning prime purge valve in counter clockwise and loading iso propanol of at least 50ml by Prime Start...

Page 98: ...uring actual analysis Solvent gas removal and filtration are necessary because they have great effect on result of analysis and maintenance of instrument 4 3 1 Degassing Solvent gas removal is perform...

Page 99: ...YL9112 4 3 3 Solvent effect on the instrument All parts of the Gradient Pump contacting with mobile phase is manufactured from 316 stainless steel ruby sapphire zirconium or fluorine carbon polymer Mo...

Page 100: ...ess out iso propanol sucked into syringe in prime purge valve and discharge at least 5ml into outlet of in line filter 5 Separate mobile phase filter assembly and block discharge hole and suction hole...

Page 101: ...click arrow button to move this on the right window Click red arrow button on the right bottom side and select the pressure unit Double click YL911x pump on the right window and check IP address of p...

Page 102: ...esume Idle If you click this button the pump goes to idle state Prime Start If you click this button the pump runs at high speed with prime pump to fill the solvent into the lines This function works...

Page 103: ...ump stops Diagnosis It is displayed during self test 4 4 3 Method Setup In this window edit time program table and setup the pump status during idle state YL9112 Click Options button to setup Max and...

Page 104: ...is opposite when you use with the other device change it on the External In Out Set 4 4 4 Error message If there are errors on the pump caused by pressure limit control value range and leak the pump s...

Page 105: ...loride Liquid Chlorine Methylene Chloride Tetrahydrofuran Teflon PTFE Dimethyl Formamide Diethylamine SS316 Phosphoric Acid Conc Rm Temp 3 Do not install instrument where corrosive gas is generated or...

Page 106: ...seal with seal insertion removal tool and insert new high pressure seal prepared at that place using seal insertion removal tool in the same manner Direction of seal should be such that the direction...

Page 107: ...ear is not well observed visually so care should be taken when observing it 1 Loosen tubing of inlet check valve and outlet check valve of pump head 2 Press pump head to main body of instrument by han...

Page 108: ...dge to remove the nitric acid used for cleaning 5 Assemble loosened check valve in the reverse order 5 5 Replacement of low pressure seal Wearing of the low pressure seal is caused when pump has been...

Page 109: ...head into parts in the same manner as seal change process of 5 2 wash it with ultrasonic cleaner and assemble each part again At this time the high pressure seal is damaged so replace it with new one...

Page 110: ...e is slippery separate it with tubing held avoiding slippage using 1000 sand paper 2 In case of performing ultrasonic wave cleaning wash head part by ultrasonic cleaner with 10 nitric acid solution fo...

Page 111: ...perly run the pump using iso propanol for 30 minutes and the plug the outlet of pump and keeps clean the solvent filter Especially after use buffer solution run the pump using 100 water first then iso...

Page 112: ...ows into head part Perform prime purge again Take measures so that air separation condition of solvent may be good and perform prime purge again Wash or replace check valve Remove oil in head sufficie...

Page 113: ...l Pump is not operated after input of power Power voltage is unstable or low Strong induction voltage is generated in the surroundings Use stable proper DC power source Close induction power source in...

Page 114: ...YL9100 HPLC System YL9120 UV Vis Detector USER MANUAL...

Page 115: ...f communication cable Chapter 3 Operation 8 3 1 YL Clarity Chromatograph software 8 3 1 1 Installation of UV Vis detector 8 YL9120 3 1 2 Device Monitor 10 3 1 3 Method Setup 11 13 3 1 4 Error message...

Page 116: ...terminal 5 Fig 2 Diagram of Remote and Marker input 5 Fig 3 Diagram of Remote and Marker output 6 Fig 4 Connection of communication cable 7 Fig 5 Front parts of YL9120 UV Vis detector 15 Fig 6 Tungste...

Page 117: ...by the blazed holographic concave grating 3 Better designed flow cell assembly by compensated RI effect 4 Improved baseline stability with heat exchange effect YL9120 5 Great Usability by mounting lam...

Page 118: ...rgy 19 Communications LAN 20 Power up Diagnostics Optics and electronic diagnostic routine 21 Power up Wavelength Verification Automatic on power up via internal holmium filter and D2 lamp 22 5 Point...

Page 119: ...20 temperature with variation 5 with and 60 humidity YL9120 2 Where no direct and straight sunshine 3 Plain floor without carpet 4 Where having spare space of 20cm or more 5 Where there are 10 or les...

Page 120: ...uld be connected as short as possible It is the ideal that you connect the tubing directly between these two ends If this is not possible you should use a minimum length of narrow bore 0 010 inch I D...

Page 121: ...t and marker input output Note Please do not connect wires between cables at your discretion If you want to connect with the other instrument please check input output information and confirm with the...

Page 122: ...MARK IN To control event program or operate additional operation MARK OUT To control time event program output READY IN To change error state and stop operation if there is a input READY OUT When YL91...

Page 123: ...YL9120 UV Vis Detector YL9120 Fig 4 Connection of communication cable Note The LAN HUB used for cable connection on the PC must use switching mode module 7...

Page 124: ...nication is connected LED blinks during connection READY RUN LED turns ON before analysis LED blinks during analysis ERROR LED turns ON if there is error 3 1 YL Clarity Chromatograph software 3 1 1 In...

Page 125: ...YL9120 UV Vis Detector Double click YL9120 UV Vis detector on the right window and check IP address of the detector YL9120 9...

Page 126: ...itor window pops up as below In this window you can control the UV Vis Detector and monitor instrument status as like lamp on off wavelength selection sampling rate etc The Device Monitor shows the st...

Page 127: ...0 Reset To release the UV Vis detector status from the error Status message Initializing It is displayed during initialization Ready It is displayed when the pump is ready Run It is displayed during...

Page 128: ...peak width is wider Auto Zero To change the absorbance to zero Auto Offset To prolong the baseline by adjustment automatically If either wavelength or polarity is changed Data Filter To select which f...

Page 129: ...ime Table choose lamps on off and edit the time table such as wavelength A B CutFilter Polarity etc according to time programming YL9120 3 1 4 Error message If there is a leak from the detector it sto...

Page 130: ...replaced to new one Generally you have to consider the lamp exchange when the light intensity of reference energy is less than 50 nA 4 1 1 How to remove the D2 lamp 1 Make sure that the power cord is...

Page 131: ...p to the mount and never attempt to rotate or move the lamp up or down in the mount The lamp Is provided as a pre aligned assembly D2 lamp assembly Fig 5 Front parts of YL9120 UV Vis detector 4 2 Tung...

Page 132: ...CAUTION The light of Tungsten lamp can damage eyes and skin Always disconnect the power cord before working in the vicinity of the lamp The W lamp gets quite hot Care must be taken while handling it t...

Page 133: ...4 3 Flow cell cleaning We do not recommend disassembly of the flow cell assembly for routine cleaning purposes Most of the cell assembly can be adequately cleaned by flushing with several milliliters...

Page 134: ...h power from the AC line Sample cell windows are contaminated Flush cell with solvents methanol acetone water nitric 6N acid water and check for leaks Sample input line has a leak Check all lines from...

Page 135: ...h UV absorbers that are bleeding replace column impure solvent is equilibrating with the column replace solvent with more pure grade switch to a longer wavelength so that background absorption does no...

Page 136: ...YL9100 HPLC SYSTEM YL9130 COLUMN COMPARTMENT USER MANUAL...

Page 137: ...6 Chapter 3 Operation 7 3 1 Before Start 7 3 2 YL Clarity Chromatograph software 7 3 2 1 Installation of column compartment 7 3 2 2 Device Monitor 8 3 2 3 Method setup 9 Chapter 4 Maintenance 12 Figur...

Page 138: ...y 0 05 C 3 Temperature accuracy 0 5 C 4 Temperature precision 0 1 C 5 Temperature programs 40 Steps 6 Column capacity Total 4 columns max 4ea of 300mm columns 7 Column switching One automatically 6 po...

Page 139: ...al layers of buffer material and fill the bottom top and all other sides of pump with buffer material in order to make pump endure shock or vibration during transportation Standard configuration of YL...

Page 140: ...umn compartment emits heat from the rear side keep distance 10cm from the wall 2 2 Connection of power YL9130 Confirm supplying voltage electric power socket and type of connector are correct Before c...

Page 141: ...re If you configure the YL9130 column compartment with switching valve use fittings supplied with system Followings are valve configurations Fig 1 Configuration of injection valve Fig 2 Configuration...

Page 142: ...ollect data at the moment of injection Notice Please do not connect wires between cables at your discretion If you want to connect with the other instrument please check input output information and c...

Page 143: ...ment MARK IN To control event program or operate additional operation MARK OUT To control time event program output READY IN To change error state and stop operation if there is a input READY OUT When...

Page 144: ...communication is connected LED blinks during connection READY RUN LED turns ON before analysis LED blinks during analysis ERROR LED turns ON if there is error 3 2 YL Clarity Chromatograph software 3 2...

Page 145: ...k YL9130 column compartment on the right window and check IP address of pump Click Detect button to check the connection 3 2 2 Device Monitor After configure the pump on the configuration window log i...

Page 146: ...lumn compartment Diagnosis To self test of instrument Reset To release the column compartment status from the error YL9130 Status message Instrument State To display the present status Act Set Temp To...

Page 147: ...Column compartment Min Temp Max Temp Set the minimum and maximum temperature The initial temperature and temperature on the Time Table are limited by these temperatures If the actual temperature is ov...

Page 148: ...ut method set the time of maintaining output signal The setup range is 100 100000 mSec YL9130 Time Table It is a table to program temperature Marker output Valve position according to time The tempera...

Page 149: ...erating temperature Solvent Leak Solvent leaks Remove the leaks on the leak sensor and find the leak position The followings are the remedy for problem Problem Cause How to fix Actual temp is much hig...

Page 150: ...YL9100 HPLC SYSTEM YL9160 PDA Detector USER MANUAL...

Page 151: ...remote cable 6 2 5 Connection of communication cable 8 Chapter 3 Operation 9 3 1 YL Clarity Chromatograph software 9 3 1 1 Installation of PDA Detector 9 3 1 2 Device monitor 10 Chapter 4 Maintenance...

Page 152: ...gh inner pressure cell assembly having RI compensated heat exchange 3 Reduced drift level by improved heat exchange 4 Improved user access by front mounting of lamps and cell 5 External valve controls...

Page 153: ...Function Available 18 Trigger Input 2ch 19 Communication TCP IP 20 Simultaneous Data Channel 8 or Full Scan 21 Filtering Bessel RC Aver 0 01 10Hz Selection of cells Microbore Analytical Semi prep Path...

Page 154: ...All parts are very precise materials so it is prohibited for a user to open the optics for repair Optics should be opened by a trained service engineer only Only remained lights after absorbed by samp...

Page 155: ...h 20 temperature with variation 5 with and 60 humidity 2 Where no direct and straight sunshine 3 Plain floor without carpet 4 Where having spare space of 20cm or more 5 Where there are 10 or less volt...

Page 156: ...connected as short as possible It is the ideal that you connect the tubing directly between these two ends If this is not possible you should use a minimum length of narrow bore 0 010 inch I D connect...

Page 157: ...for remote input output and marker input output Note Please do not connect wires between cables at your discretion If you want to connect with the other instrument please check input output informatio...

Page 158: ...utosampler or external valve automatic running is available START OUT If the signal input on the START IN terminal the signal pulse output through this port It can be used for synchronization of remot...

Page 159: ...n cable YL9160 PDA detector provides TCP IP internet protocol as a standard The IP address of YL9100 series is 10 10 10 60 if DIP SW settings on the rear side are On position If you change the IP addr...

Page 160: ...f communication is connected LED blinks during connection READY RUN LED turns ON before analysis LED blinks during analysis ERROR LED turns ON if there is error 3 1 YL Clarity Chromatograph software 3...

Page 161: ...ctor to pop up Setup window Check IP address and add acquisition channels and PDA module 3 1 2 Device monitor After setting the PDA log in the software then following device monitor windows will come...

Page 162: ...n error happens D2 On Off Turn on off D2 lamp W On Off Turn on off Halogen tungsten lamp Status Display of YL9160 status Ready Initializing Run or any errors Sampling Rate Display acquisition rate sel...

Page 163: ...e for the acquisition date from 0 1 9 9 Filter Type select filter type applied to the response time Drift Comp select if want to compensate drift If selected automatically compensate the drift within...

Page 164: ...alf of the original intensity the lamp should be replaced to new one Generally you have to consider the lamp exchange when the light intensity of reference energy is less than 50 nA 4 1 1 How to remov...

Page 165: ...e lamp up or down in the mount The lamp Is provided as a pre aligned assembly D2 lamp assembly Flow cell assembly Tungsten lamp assembly Fig 5 Front of YL9160 PDA Detector 4 2 Tungsten lamp W The repl...

Page 166: ...t from burning Always allow the lamp to cool before removing it 3 Disconnect the Tungsten lamp from the detector by gently pulling it straight back toward you DO NOT twist the connector while pulling...

Page 167: ...ppropriate solvent We recommend the following solvents for this purpose 1 Methanol 2 Tetrahydrofuran 3 Methylene Chloride 4 HPLC Grade Water 5 6 N Nitric Acid followed by flushing with HPLC Grade Wate...

Page 168: ...rease flow rate and or back pressure on cell Recorder or integrator is grounded and is causing a ground loop problem Check recorder with voltmeter to see if either of the signal inputs is grounded to...

Page 169: ...YL9100 HPLC System YL9170 RI Detector USER MANUAL...

Page 170: ...les 4 Chapter 3 Operation 5 3 1 YL Clarity Chromatograph software 5 3 1 1 Installation of YL9170 RI detector 5 3 1 2 Device Monitor 6 3 1 3 Method Setup 8 Chapter 4 Maintenance 9 4 1 Adjusting the opt...

Page 171: ...ve or Semi micro application Option 1 2 Specifications 1 Detection method Deflection 2 RI Range 1 00 to 1 75 RIU 3 Typical flow rate 0 2 3 0 ml min 4 Noise 5 x 10 10 RIU 5 Auto zero range 40 x 10 5 RI...

Page 172: ...equirement of YL9170 RI Detector 1 Room with 20 temperature with variation 5 with and 60 humidity 2 Where no direct and straight sunshine 3 Plain floor without carpet 4 Where having spare space of 20c...

Page 173: ...f tubing between the inlet of flow cell assembly and outlet of column should be connected as short as possible It is the ideal that you connect the tubing directly between these two ends If this is no...

Page 174: ...the sample cell of the detector Connect the capillary coming from the HPLC column with the INLET port of RI Detector Use the stainless steel capillary tubing with the Teflon tubing and connect the tub...

Page 175: ...ALITY LED indicates the polarity of signal output About 2 minutes later after power up you can control and operate YL9170 RI detector by YL Clarity 3 1 YL Clarity Chromatograph software 3 1 1 Installa...

Page 176: ...e Monitor After configure the RI detector on the System Configuration window log in to open main control window On the main control window click Device monitor and then Device Monitor window pops up a...

Page 177: ...s the temperature of cell optical balance and out signal as well YL9170 Descriptions of control buttons Purge Start Stop flushing cells with solvents Temp On Off Set up On Off for the cell temperature...

Page 178: ...table and setup the detector status during idle state Range Set up for the maximum limit of voltage range Sample Rate Set up for the sample rate Autozero Set up for autozero at a data acquisition Tem...

Page 179: ...ecorder The recorder output is set to 1 by default Autozero Use of this button autozeros the detectors signal Purge Pressing this button flow and reference cell will be flushed with solvent LED is hig...

Page 180: ...ror During the procedure you can follow the changes by observing the change of the detector signal or more detailed by observing the difference and sum voltage To adjust the optical system follow the...

Page 181: ...ing steps Switch off the instrument Unplug the main cable to prevent electrical shock NOTE Some operations during adjustment have to be done when the instrument is in operation So you need to reconnec...

Page 182: ...ponents Use a voltmeter to check the lamp voltage at the test points on the circuit board The label of this test point depends on the version of this board lampe and GND or LampeULmp and GND The lamp...

Page 183: ...e light source This is illustrated in the following figure Slit aperture NOTE The projected picture at the light sensor should be a sharp one The full height of the photodiodes has to be illuminated b...

Page 184: ...e performing this test 1 Press the Purge button Make sure the purge mode is activated 2 Use a disposable syringe to press approx 5mL distilled water through the flow cell 3 The display will show the c...

Page 185: ...replacement of the valve If the valve which is used to switch the path of the mobile phase in the normal mode and purge mode needs to be replaces follow the following steps Swtich off the YL9170 and...

Page 186: ...the optical bench 8 Open the inner black housing 9 Loosen the two hex screws holding the slit aperture 2 and remove the aperture carefully 10 Use a flash light to check the flow cell for contaminants...

Page 187: ...to the service mode where the temperature is displayed with three digit e g 35 012 o C 2 The temperature sensor will be detected automatically when the instrument is switched on 3 If the heating is s...

Page 188: ......

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YL Instruments YL9100 HPLC, User Manual

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Summary of Contents for YL9100 HPLC

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