YL Instruments YL9100 HPLC, User Manual

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YL9170 RI Detector
4-3. Checking Sum and Difference voltages
This chapter describes the check of the sum and difference voltages. These voltages result from different
intensities reaching the sample side and the reference side of the light sensor.
To perform the check follow the steps listed below:
Make sure the instrument is switched on and both chamber of the flow cell are filled with distilled water
wthout bubbles. In this case the optical balance should be 0
±
20.0%%. To ensure a stable temperature
the instrument should be swtiched on for several hours before performing this test.
1) Press the Purge button. Make sure the purge mode is activated.
2) Use a disposable syringe to press approx. 5mL distilled water through the flow cell.
3) The display will show the current detector signal.
4) Activate the service mode. The display will change to the following
Temp: +0023.500
o
C
Opt. Bal: +0013.400 %%
Signal: +0015.000
mV
ServMode >>
Menu,
↵
5) Press the MENU button until the status line changes to ViewFine. Now press the ENTER button.
The display will show four values.
Check Source Unit
↵
0044 1636 0840 0796
Signal: + 250.668 mV
ServMode Viewine
To find out which value is displayed in which column press the ENTER button. The display will change to
the following view:
Check Source Unit
Diff Summ Smpl Rfrn
Signal: + 250.668 mV
ServMode Viewine
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