
15
2. Before Using
Preparation before operation
Time lag
a
b
c
℃
Time
①
②
③
④
a= Preset sterilization time
b= Time lag
c= sterilization time defined by the
Japanese Pharmacopoeia
Temperature inside the chamber
Saturated
vapor
temperature
Water temperature
・
Preset sterilization temperature:121
℃
・
Room temperature: 25
℃
(using conical flask)
When sterilizing liquid samples, a time lag (b) is made between the
temperature inside the chamber and actual temperature of liquid by
the time when the liquid temperature reaches the preset sterilization
temperature. For this reason, a longer time than defined by the
Japanese Pharmacopoeia (c) is required to completely sterilize the
samples. Consequently, the actual preset sterilization time (a) should
be set to be extended
The right table shows the time lag between the temperature inside
the chamber and actual temperature of liquid (water). The table
below shows the temperature rise and cooling time with no load
(liquid).
Time lag
Load
SM520/820
500ml
12min.
1000ml
15min.
2000ml
16min.
3000ml
20min.
4000ml
25min.
5000ml
30min.