14
2. Before Using
Preparation before operation
Reference data
Sterilizing operation using disposal bag for biochemically dangerous object
①
Open the opening of sterilization bag so the vapor can be easily entered into
it. Secure the bag with a wire rack so it should not fall down during operation.
②
The height of bag should be about two-thirds of chamber. If it is too high, the
vapor cannot be easily entered into it, or it blocks the vapor outlet at the upper
part of chamber, which may cause insufficient sterilization.
③
The preset temperature should be lower than the upper temperature limit of
bag.
④
The preset time varies depending on the quality and quantity of sterile samples. Refer to the
following data for the preset time.
During liquid sterilization
①
If the sterilization temperature exceeds the boiling temperature of the liquid, (under atmosphere
pressure) the boiling point of the liquid should not be lower than water boiling point minus 5
℃
(under atmosphere pressure, if the water boiling point is 100
℃
, the boiling point of the liquid
should not be lower than 95
℃
).
During sterilization, the liquid should be encapsulated by vessel, and its volume should not exceed
80% of the vessel (to prevent gas expansion due to boiling which may result in explosion).
Reference example of SM520 model at room temperature of 25
℃
Sterile sample
Sterilization temperature
Sterilization time
Note
Gauze
121
℃
30 min.
Dry gauze 2.8*3m*5
Petri dish
121
℃
40 min.
30 Petri dish with a cover
The data above, however, is used as reference. The actual sterile condition varies depending on the
characteristics and quantity of samples or type of vessels to be used. Confirm the sterile condition by
using the biological indicator or chemical indicator.
The use of the sterilization test card (amlessories)
・
When use the sterilization test card, please insert card in the center of the sample.
・
Opeating temp.: 121
℃
(attached sterilization test card is 121
℃
)
・
After the operation, please confirm the sterilization state at the sterilization test card
0000
日付
滅菌条件が完全なときには の中に
”滅菌済”が黒褐色で表示されます。
ＨＰ滅菌カード（オートクレーブ用）
SS 121-20
滅菌済
If poor sterilization, shallow dark brown
灭菌済
is
ahown in
000000
日付
滅菌条件が完全なときには の中に
”滅菌済”が黒褐色で表示されます。
ＨＰ滅菌カード（オートクレーブ用）
SS 121-20
滅菌済
If normal sterilization, clear dark brown
灭菌済
is
ahown in
000000
Sterilization
bag