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6
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Operation
Page 92
Vanquish Diode Array Detectors (VH-D10) Operating Manual
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Solvents with a low boiling point can evaporate in the warm flow
cell, thus degrading the performance of the flow cell. Consider that
the combination of temperature and pressure of the eluate in the
flow cell can prevent boiling of the eluate.
•
Particulate matter from the eluate, system modules and
components can deposit in the flow cell and clog it. This can lead to
an instable baseline behavior, such as increased noise, or a blockage
and a destruction of the flow cell. To avoid this, observe the
following:
♦
Use the flow cell only with a column or a filter frit connected in
the flow path before the flow cell.
♦
Use highly pure solvents, such as of LC/MS-grade, only.
♦
If capillaries are removed from the flow cell inlet and outlet,
install the plugs supplied with the flow cell to close the ports.
•
When system operating parameters, such as temperature or
pressure, change in the flow cell, salt from eluates with high salt
contents, such as buffers, may crystallize in the flow cell and cause
precipitations.
♦
Never leave eluates with high salt content in the flow cell
without flow. Flush the flow cell regularly when using
concentrated salt solutions. Monitor the backpressure.
♦
Make sure that buffer salts remain soluble also with the highest
applied content of organic solvent.
•
The flow cell is sensitive to thermal changes in the environment. If
the doors are opened during analysis, the thermal changes may lead
to a baseline drift.
•
During long analysis breaks
Close the shutter in the light path before the flow cell to protect the
flow cell from the UV light.
•
Do not connect a switching valve, as for example used in a fraction
collector, in the flow path after the flow cell.
Summary of Contents for Vanquish VH-D10
Page 4: ...Contacting Us Page 4 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 44: ...4 Unpacking Page 44 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 80: ...5 Installation Page 80 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 110: ...6 Operation Page 110 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 162: ...7 Maintenance and Service Page 162 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 190: ...11 Appendix Page 190 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 191: ...12 Index Vanquish Diode Array Detectors VH D10 Operating Manual Page 191 12 Index...
Page 195: ...12 Index Vanquish Diode Array Detectors VH D10 Operating Manual Page 195...