ThermoFisher Scientific Vanquish VH-D10, Operating Manual

Page: 91 / 196

6 • Operation
Vanquish Diode Array Detectors (VH-D10) Operating Manual Page 91
TIP The Chromeleon software supports procedures for automatically
starting a chromatography system in the software ( Smart Startup ). The
startup procedure includes the operations for system equilibration. For
details, refer to the
Chromeleon Help
.
TIP When you start the data acquisition or perform an autozero, the
shutter in the light path before the flow cell is opened automatically.
After the data acquisition, close the shutter, if required.
6.5.1 Guidelines for Use of LightPipe Flow Cells
NOTICE—Sensitive LightPipe Flow Cells
LightPipe flow cells are highly sensitive to damage. Handle LightPipe
flow cells with care. Observe the following guidelines for use of the flow
cell.
•
Observe the specified maximum temperature limit and pressure
limit for the flow cell. See the specifications for LightPipe flow cells
in
section 9.1.2, page 177 .
• If your application requires column temperatures above the
specified maximum limit, install and use a post-column cooler in the
flow path before the flow cell.
•
If the pump flow is interrupted, take appropriate measures to
protect the LightPipe flow cell.
♦
If the lamp is on, close the shutter in the light path in front of
the flow cell, or turn off the lamp to protect the flow cell from
the light of the lamp and to prevent the growth of algae.
♦ Never leave any substances in the flow cell without flow. Avoid
leaving particularly any aggressive solvents in the flow cell for a
longer time.
♦
Without flow, air bubbles in the flow cell can accelerate the
deposition of substances. Avoid the deposition of substances in
the flow cell as a result of liquids with air bubbles. Never leave
air bubbles in the flow cell without flow. If the pump flow is
stopped for a longer time, fill the flow cell with a high purity
solvent.