ThermoFisher Scientific Vanquish VH-D10, Operating Manual

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6 • Operation
Page 100 Vanquish Diode Array Detectors (VH-D10) Operating Manual
6.7 Optimizing the Performance of the Detector
This section provides information for best performance of the detector
and gives hints on what you can do to optimize the performance further.
For information on the default parameters, see section 6.6 Important
Operating Parameters, page 94 .
6.7.1 General Guidelines
Consider the following guidelines for optimization of the detector
performance:
•
The recording of up to 10 signal channels at individual wavelengths
enables a selective detection with narrow bandwidths.
In a selective detection, the UV spectrum for a particular species is
recorded. Determine an appropriate absorbance maximum.
Avoid the wavelength range where the solvents absorb strongly (for
example, below 220 nm for methanol and below 210 nm for
acetonitrile).
•
Consider the influences of the parameters on the detection (see
section 6.7.2 Overview of Optimization Parameters, page 101 ).
• Ignite the UV lamp only if required. Igniting the UV lamp frequently
reduces the lifetime of the lamp. In addition, some time is required
for the detector to stabilize after a (re-)ignition of the lamp.
•
Keep the detector doors closed during operation to avoid exposure
of the flow cell to thermal changes in the environment that may lead
to a baseline drift.
•
Ensure that the operating conditions are suitable. This includes:
♦
Stable environmental conditions, such as a stable temperature
♦
No air drafts
♦
No vibrations or mechanical shocks caused by external sources
♦
Stable backpressure and correct waste line setup