7
•
Maintenance and Service
Vanquish Diode Array Detectors (VH-D10) Operating Manual
Page 121
7.5
Performing a Wavelength Validation
A holmium-oxide glass filter is used to validate the wavelength accuracy.
The filter can be moved into the light path between the lamp and the
flow cell for the wavelength validation. The detector determines the
absorption maxima of the filter and compares them to the nominal
holmium oxide values that are stored in the detector firmware.
The accuracy is verified for the wavelengths that are stated in the
declaration of conformity for the holmium-oxide glass filter. For
information about the declaration, see
section 11.1.4 NIST Compliance,
page 188
A wavelength validation can also be performed with an external
standard, such as a pyrene solution. In this case, an accuracy of ± 1 nm
can be achieved.
When
•
During initial operation of the detector
•
After the flow cell has been exchanged
•
After a lamp has been replaced
Preparations
Before you start the wavelength validation, observe the following notes:
•
Ensure that the baseline is sufficiently stable. The baseline may
become unstable, for example, if the solvent composition has been
modified, or if air bubbles are present in the light path.
•
Verify that the solvent flowing through the cell is not strongly
absorbing in the wavelength range of the holmium-oxide glass filter
that is to be verified.
This problem occurs, for example, if the cell is filled with a mixture of
96% hexane and 4% ethyl acetate. Thermo Fisher Scientific
recommends using degassed LC/MS-grade water.
•
Allow the lamp to warm up and reach operating temperature. The
lamp should be running for at least 1 hour before you start the
wavelength validation. A lamp spectrum changes significantly during
the first few minutes after a lamp has been turned on.
Summary of Contents for Vanquish VH-D10
Page 4: ...Contacting Us Page 4 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 44: ...4 Unpacking Page 44 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 80: ...5 Installation Page 80 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 110: ...6 Operation Page 110 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 162: ...7 Maintenance and Service Page 162 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 190: ...11 Appendix Page 190 Vanquish Diode Array Detectors VH D10 Operating Manual...
Page 191: ...12 Index Vanquish Diode Array Detectors VH D10 Operating Manual Page 191 12 Index...
Page 195: ...12 Index Vanquish Diode Array Detectors VH D10 Operating Manual Page 195...