iSort
™
Automated Cell Sorter User Guide
71
11.
On the Drop Delay Calibration screen, verify the presence of the calibration
beads in the fluorescence scatter plot.
12.
Ensure that the event rate counter located above the scatter plot
registers an event rate of between 100 and 500, then press
Continue
.
13.
When prompted, collect the sorted puddles for the first calibration point (you
will collect a total of eight calibration points):
a.
Wipe off a microscope slide with a laboratory wipe to make sure it is clean
and free of dust and other particulate matter.
b.
Hold the slide below Region 1 (left collection region) of the closed sort
drawer until the instrument deposits three small puddles on the slide.
c.
Press
Continue
to proceed with examining the puddles for the presence of
the calibration beads.
Summary of Contents for invitrogen iSort Series
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