88
iSort
™
Automated Cell Sorter User Guide
7.
Shut Down
Daily Shut Down
You must perform the Shut Down procedure after the last Analysis or Sort of the
day, then remove the nozzle tip for cleaning and storage. The Shut Down
procedure sanitizes the system fluidics with 10% bleach solution, flushes it with a
detergent solution, then rinses it with deionized water to complete the cleaning
process. It then turns off the fluid stream, charging plates, and the laser. The entire
procedure takes approximately 30 minutes to complete.
Required solutions
•
10% bleach solution
•
Attune
™
Wash Solution (Cat. No. A24974) (wash solution in Step 5)
•
Deionized water
IMPORTANT!
10% bleach is defined as a 1:10 dilution (1 part bleach to 9 parts
water) of 5.25% sodium hypochlorite in deionized water. This gives a final
concentration of 0.5% sodium hypochlorite equivalent to 5000 ppm of available
chlorine. We recommend using laboratory-grade bleach. Avoid bleach with
additives (such as perfumes).
Run the Shut Down
procedure
1.
On the Home screen, press the
Shut Down
button.
The instrument displays the Confirm Shut Down dialog.
Summary of Contents for invitrogen iSort Series
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