26
iSort
™
Automated Cell Sorter User Guide
Run Initial Cleaning
Overview
Initial Cleaning
must be performed before running the first analysis or sort of the
day. The initial cleaning process takes approximately 10 minutes. It is not necessary
to repeat the initial cleaning cycle before subsequent analysis or sorts. However, if
the instrument remains idle for more than 30 minutes, it will prompt you to perform
the initial cleaning procedure again to prepare the sample line for further analysis
or sort runs. This repeat process is optional, but recommended for improved sort
performance and to minimize clogs.
Required solutions
•
Sheath fluid (1X PBS, flow cytometry grade)
•
Deionized water
Run Initial Cleaning
1.
On the Home Screen, press the
Maintenance
button to open the
Maintenance screen.
2.
On the Maintenance screen, press
Cleaning Cycles
, then select
Initial Cleaning
.
3.
When prompted, press
Continue
to start
the fluidic flow and begin the initial
cleaning process. Some noise will be
generated as the fluidics flush begins.
Summary of Contents for invitrogen iSort Series
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