ThermoFisher Scientific invitrogen iSort Series, Manual

Page: 61 / 140

iSort
™
Automated Cell Sorter User Guide 61
Sheath Fluidics Cleaning
Overview Sheath Fluidics Cleaning is used for decontaminating the fluidics lines of the
iSort ™ Automated Cell Sorter. Running the sheath fluidics cleaning also requires
you to change the 0.2-µm sheath fluid filter. The entire procedure takes
approximately 2 hours to complete.
Note:
We recommend that you run the sheath fluidics cleaning procedure at least
once every six months or as needed to decontaminate the fluidics lines. If you plan
to ship your instrument for service, you must also perform the decontamination
procedure.
A noticeably high background in the acquired data can indicate bacterial or fungal
contamination in the fluidics lines, which can result from not following sterile
technique when handling cell samples. Another source of contamination may be
the bulk fluidics despite the presence of filters in the fluidics lines of the system.
Required materials •
1 L of 10% bleach solution (cleaning solution used in Step 8 of the protocol,
page 63)
•
Sheath fluid (1X PBS, flow cytometry grade)
•
Deionized water
•
0.2-µm sheath inlet filter (Cat. No. A33307)
•
Sheath strainer (Cat. No. A33311)
IMPORTANT!
10% bleach is defined as a 1:10 dilution of 5.25% sodium hypochlorite
in deionized water. This gives a final concentration of 0.5% sodium hypochlorite
equivalent to 5000 ppm of available chlorine. We recommend using laboratory-
grade bleach. Avoid bleach with additives (such as perfumes).
Run Sheath
Fluidics Cleaning
1. On the Maintenance screen, press Cleaning Cycles , then select Sheath Fluidics
Cleaning .