ThermoFisher Scientific invitrogen iSort Series, Manual

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94 iSort
™
Automated Cell Sorter User Guide
9. Instrument care and maintenance
Planned maintenance schedule
To ensure reliability of the iSort ™ Automated Cell Sorter, you must perform basic preventative maintenance
procedures on a regular basis. In addition, certain maintenance and parts replacement procedures must be
performed by the Thermo Fisher Scientific field service engineers (FSE) annually. The following table lists
the routine maintenance procedures that keep the iSort
™
Automated Cell Sorter in good working condition.
For detailed instructions on how to perform a specific maintenance function, see pages 96–118.
Procedure Frequency Materials required User FSE
Inspect and wipe down bottle
and fluidics compartment area
for drips
Daily Kimwipes
™
laboratory tissues (or other soft,
lint free cloth)

Perform initial cleaning Daily and when
prompted
Sheath fluid (1X PBS, flow cytometry grade)
(Cat. No. A1286301), deionized water

Shut down the system Daily 10% bleach solution, Attune
™
Wash Solution
(Cat. No. A24974), deionized water

Remove and clean the nozzle
tip, store it in cleaning solution Daily 5% Contrad
™
70 detergent solution (cleaning
solution), deionized water, ultrasonic bath
(sonicator), iSort
™
Nozzle Tip cover (Cat. No.
A33411)

Remove and clean the
deflection plates Daily Kimwipes
™
laboratory tissues (or other soft,
lint free cloth), isopropyl alcohol or ethanol

Clean the sort chamber Between sorts Kimwipes
™
laboratory tissues (or other soft,
lint free cloth), deionized water

Clean the SIP (sample input
port) Between sorts Kimwipes
™
laboratory tissues (or other soft,
lint free cloth)

Perform sample line cleaning Between sorts 10% bleach solution, Attune
™
Wash Solution
(Cat. No. A24974), deionized water

Perform flow rate calibration As needed Attune
™
Performance Tracking Beads (Cat.
No. 4449754), 5% Tween
™
20 in deionized
water, 10X PBS (Cat. No. 70011044), Sheath
fluid (1X PBS, flow cytometry grade) (Cat.
No. A1286301), deionized water

Perform drop delay calibration As needed Attune
™
Performance Tracking Beads (Cat.
No. 4449754), 10X PBS (Cat. No. 70011044),
Sheath fluid (1X PBS, flow cytometry grade)
(Cat. No. A1286301), deionized water,
microscope slide (Cat. No. A36699),
fluorescent microscope
 
Perform gimbal adjustment As needed Sheath fluid (1X PBS, flow cytometry grade)
(Cat. No. A1286301)
