iSort
™
Automated Cell Sorter User Guide
27
4.
When prompted, place an empty 12 × 75-mm sample tube into the sample tube
holder in the down position.
5.
Press
Continue
to begin the back flush component of the initial cleaning cycle,
which removes clogs from the sample lines.
6.
When prompted, place a fresh 12 × 75-mm sample tube with 3 mL of sheath
fluid into the sample tube holder, then move the sample tube holder to the up
position.
7.
Press
Continue
to begin the fluidic flush component of the initial cleaning
cycle, which washes the sample lines with sheath fluid and is designed to
prevent carryover between samples.
8.
At the completion of the initial cleaning, a prompt will confirm “Initial
Cleaning Complete”. Proceed to Performance Test (page 28) or Analyze and
Sort (page 31).
Summary of Contents for invitrogen iSort Series
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