Thermo Scientific NanoDrop One, User Manual

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10
Measure Protein BCA
Thermo Scientific NanoDrop One User Guide 93
• After the minimum number of standards has been measured for the selected curve type,
the message “Invalid Curve” changes to “Valid Curve.” (This occurs even when additional
standards have been defined but not yet measured.) If the “Invalid Curve” message
remains after all entered standards have been measured, try:
– selecting a different curve type
– remeasuring standards using the correct standard material
Valid Curve indicator : This is only an indicator that the required minimum number of
points has been established for the selected curve type. It does not validate the integrity of
the curve. For example, additional standards may be required to cover the expected assay
concentration range.
To measure Protein BCA standards and samples
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the instrument
arm and clean the upper and lower pedestals. At a minimum, wipe the pedestals with a new
laboratory wipe. For more information, see
Cleaning the Pedestals .
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will flow into
the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently
damage the quartz fiber optic cables.

To measure Protein BCA standards and samples
1. From the Home screen, select the Proteins tab and tap Protein BCA .
2. Specify a curve type and number of replicates for each standard and enter the
concentration of each standard .
Tip : For this assay, we recommend setting Curve Type to “Linear”.
3. Measure blank:
– pipette 2 μL DI H
2
O onto lower pedestal and lower arm, or insert DI H
2
O
blanking cuvette into cuvette holder