Thermo Scientific NanoDrop One, User Manual

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2
Detection Limits for All Applications
10 NanoDrop One User Guide Thermo Scientific
Purified BSA by
Protein A280
IgG by Protein
A280
0.06 mg/mL (pedestal)
0.006 mg/mL (cuvette)
0.03 mg/mL (pedestal)
0.003 mg/mL (cuvette)
825 mg/mL (pedestal)
402 mg/mL (pedestal)
±0.10 mg/mL (for 0.10–10 mg/mL samples);
±2% for samples >10 mg/mL
Purified BSA by
Proteins & Labels 0.06 mg/mL (pedestal)
0.006 mg/mL (cuvette)
19 mg/mL (pedestal) ±0.10 mg/mL for 0.10–10 mg/mL samples
Protein BCA 0.2 mg/mL (20:1
reagent/sample volume)
0.01 mg/mL (1:1
reagent/sample volume)
8.0 mg/mL (pedestal)
0.20 mg/mL (cuvette)
2% over entire range
0.01 mg/mL over entire range
Protein Lowry 0.2 mg/mL (pedestal) 4.0 mg/mL (pedestal) 2% over entire range
Protein Bradford 100 μg/mL (50:1
reagent/sample volume)
15 μg/mL (1:1
reagent/sample volume)
8000 μg/mL
100 μg/μL
±25 μg/mL for 100–500 μg/mL samples
±5% for 500–8000 μg/mL samples
±4 μg/mL for 15–50 μg/mL samples
±5% for 50–125 μg/mL samples
Protein Pierce 660 50 μg/mL (15:1
reagent/sample volume)
25 μg/mL (7.5:1
reagent/sample volume)
2000 μg/mL
1000 μg/mL
±3 μg/mL for 50–125 μg/mL samples
±2% for samples > 125 μg/mL
±3 μg/mL for 25–125 μg/mL samples
±2% for samples >125 μg/mL
a
Based on five replicates (SD=ng/μL; CV=%)
Sample Type Lower Detection Limit Upper Detection Limit Typical Reproducibility
a
Note To minimize instrument error with highly concentrated samples, make dilutions to
ensure that measurements are made within these absorbance limits:
• For micro-volume measurements, maximum absorbance at 260 nm (for nucleic acids)
or 280 nm (for proteins) should be less than 62.5 A.
• For measurements with 10 mm pathlength cuvettes, maximum absorbance at 260 nm
(or 280 nm for proteins) should be less than 1.5 A, which is approximately 75 ng/μL
dsDNA.