Thermo Scientific NanoDrop One, User Manual

Page: 110 / 328

11
Measure Protein Bradford
104 NanoDrop One User Guide Thermo Scientific
Related Topics
• Working with standard curves
• Best practices for protein measurements
• Measure a Micro-Volume Sample
Tip : If using a cuvette, make sure to align cuvette light path with instrument light
path.
– tap Blank and wait for measurement to complete
– lift arm and clean both pedestals with new laboratory wipe, or remove cuvette
4. Measure reference standard:
– pipette 2 μL reference solution onto pedestal, or insert reference cuvette (reference
solution should contain none of the standard protein stock, see
Working With
Standard Curves for details)
– lower arm to start measurement (or tap Measure if Auto-Measure is off )
– lift arm and clean both pedestals with new wipe, or remove cuvette
– if Replicates setting is greater than 1, repeat measurement
5. Measure remaining standards:
– pipette 2 μL standard 1 onto pedestal, or insert standard 1 cuvette
– lower arm to start measurement (or tap Measure if Auto-Measure is off )
– lift arm and clean both pedestals with new wipe, or remove cuvette
– if Replicates setting is greater than 1, repeat measurement
– repeat substeps above for each additional standard (when specified number of
standards and replicates have been measured, a message asks whether to load more
standards or begin measuring samples)
– if finished measuring standards, tap Done (swipe left to view standard curve)
6. Measure samples:
– pipette 2 μL sample 1 onto pedestal, or insert sample 1 cuvette
– lower arm to start measurement (or tap Measure if Auto-Measure is off )
– lift arm and clean both pedestals with new wipe, or remove cuvette
– if Replicates setting is greater than 1, repeat measurement
7. When you are finished measuring samples, tap End Experiment .
8. Lift the arm and clean both pedestals with a new wipe, or remove the sample cuvette.