Thermo Scientific NanoDrop One, User Manual

Page: 61 / 328

7
Measure Protein A280
Thermo Scientific NanoDrop One User Guide 55
 To measure a Protein A280 sample
1. From the Home screen, select the Proteins tab and
tap Protein A280 .
2. Specify a sample type and baseline correction if
desired.
3. Pipette 1–2 μL blanking solution onto the lower
pedestal and lower the arm, or insert the blanking
cuvette into the cuvette holder.
Tip : If using a cuvette, make sure to align the cuvette
light path with the instrument light path.
4. Tap Blank and wait for the measurement to
complete.
Tip : If Auto-Blank is On, the blank measurement
starts automatically after you lower the arm. (This
option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new
laboratory wipe, or remove the blanking cuvette.
6. Pipette 2 μL sample solution onto the pedestal and
lower the arm, or insert the sample cuvette into the
cuvette holder.
7. Start the sample measurement:
– Pedestal: If Auto-Measure is On, lower arm; if
Auto-Measure is off, lower arm and tap Measure .
– Cuvette: Tap Measure
When the sample measurement is completed, the
spectrum and reported values are displayed (see the
next section).
8. When you are finished measuring samples, tap
End Experiment .
9. Lift the arm and clean both pedestals with a new
wipe, or remove the sample cuvette.
High concentration BSA sample
Low concentration BSA sample