Thermo Scientific NanoDrop One, User Manual

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10
Measure Protein BCA
100 NanoDrop One User Guide Thermo Scientific
Settings for Protein BCA Measurements
To show the Protein BCA settings, from the Protein BCA measurement screen, tap >
Protein BCA Setup.
Related Topics
• Instrument Settings
Note You can edit the Curve Type setting when measuring standards by changing the list
box at the top of the application measurement screen. You can edit the concentration
value for a standard from the application setup screen. After the first sample measurement,
these settings cannot be changed.
Setting Description
Curve Type Specify type of equation used to create standard curve from standard concentration values.
Available options:
– Linear : Draws the linear least squares line through all measured standards (requires
reference measurement and at least one standard)
– Interpolation : Draws a series of straight lines to connect all measured standards
(requires reference measurement and at least one standard)
– 2
nd
order polynomial : Draws the 2
nd
order least squares polynomial using all
measured standards (requires reference measurement and at least two standards)
– 3
rd
order polynomial : Draws the 3
rd
order least squares polynomial using all
measured standards (requires reference measurement and at least three standards)
Replicates Enter number of measurements of the reference or the same standard or sample that are
averaged together to produce its associated concentration value.
Note : Replicates setting cannot be changed after the first standard has been measured.
Standards Enter actual concentration value of each standard.
Note : Concentration values can be entered in any order but the standards must be measured
in the order they were entered.