White Blood Cell Depletion (WBCD) and Platelet Depletion (PLTD) Procedures
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Spectra Optia Apheresis System Operator’s Manual
Optimizing the Run
Facilitating the Flow of Replacement Fluid
During WBCD and PLTD procedures, replacement fluid is delivered by gravity. The system does not pump the
fluid into the reservoir. To facilitate the flow of the replacement fluid, perform the following steps:
•
Make sure that the IV pole from which the replacement fluid hangs is fully extended to maximize the effect of
gravity.
•
Confirm that the frangible connector on the replace line is completely broken so that it does not obstruct the
fluid flow.
•
Fill the drip chamber on the replace line so that it is nearly full to prevent the formation of bubbles in the line.
•
Remove any air bubbles you see in the replace line.
Improving the flow of replacement fluid contained in bottles
Fluid contained in bottles might not flow as well by gravity as fluid contained in bags because bottles are not
vented. If the replacement fluid you are using is contained in a bottle, perform the following steps when spiking the
bottle to help improve the flow:
1
Squeeze and hold the drip chamber on the spike on the replace line.
2
Continue squeezing the drip chamber as you spike the bottle. Do not stop squeezing the drip chamber until
you invert the bottle and see that the spike is immersed in fluid.
3
Release the drip chamber. It should fill with fluid.
Optimizing the Collection Preference
The collection preference controls the concentration of cells that flow through the collect port. You can use the up
and down arrows on the screen to increase or decrease the collection preference during the run to accommodate the
patient's condition and to achieve your procedure goals. Consider the following guidelines when adjusting the
collection preference, keeping in mind that the patient's cell counts affect the contents of the collection:
•
Use a low collection preference to increase the concentration of cells in the collect port. The system adjusts the
plasma pump flow rate so that it collects more deeply in the buffy coat. This maximizes the yield of target cells
but could increase RBC contamination of the collected product.
•
Use a high collection preference to decrease the concentration of cells in the collect port. The system adjusts
the plasma pump flow rate so that it collects more shallowly in the buffy coat. This helps to minimize RBC
contamination of the collected product but could result in a lower yield of target cells.
SpectraOptiaOpsManual.book Page 190 Thursday, May 17, 2018 11:24 AM