Spectra Optia Apheresis System Operator’s Manual
145
Mononuclear Cell (MNC) Collection Procedures
10
•
Increase the inlet:AC ratio. This causes the inlet pump flow rate to increase. However, it decreases the system’s
ability to manage anticoagulation of the extracorporeal circuit and can result in clumping in the circuit.
Monitor the run carefully as you increase the inlet:AC ratio.
•
Decrease the target run time. Make the run time the run target by entering the desired time on the run values
or run targets screen. This ensures that the run ends when required.
Transferring Plasma from the Plasma Bag into the Collection Bag
The system allows you to transfer plasma from the plasma bag into the collection bag during the run except during
a collection phase. To transfer plasma during the run, perform the following steps:
1
Touch the
End Run
menu button. The end run tabs appear.
2
Touch the
Plasma
tab.
3
Follow the instructions on the screen to put the collection bag lower than the plasma bag.
4
Touch
Start Transfer
. The plasma valve moves into the neutral position and the RBC valve moves into the
collect position.
5
Allow the plasma to flow from the plasma bag into the collection bag.
6
When the transfer is complete, touch
Resume Run
, or if the run targets have been attained and you want to
end the run, touch
End Transfer
. The plasma and RBC valves move back to the return position. The not
equal icon (
) appears next to the plasma bag volume and the collection bag volume on the screens
indicating that the current volumes may be different from the displayed volumes.
Transferring plasma when a collection phase is ready to start
If the chamber is full and a collection phase is ready to start, the system pauses the pumps and delays the collection
phase. To complete the transfer, follow the instructions under “Transferring Plasma from the Plasma Bag into the
Collection Bag” above, beginning with step 4. To cancel the transfer and allow the collection phase to occur, touch
the go back button.
Caution:
When transferring plasma from the plasma bag to the collection bag during an MNC collection procedure, you
must put the collection bag lower than the plasma bag as indicated in the instructions on the screen. Otherwise, cells in the
collection bag can flow into the plasma bag.
Note:
If you obtained a product sample before you transferred plasma into the collection bag, consider taking a new
sample. The additional volume you added to the collection bag could affect the cell concentration.
SpectraOptiaOpsManual.book Page 145 Thursday, May 17, 2018 11:24 AM
