Supplementary Information
270
Spectra Optia Apheresis System Operator’s Manual
Adding Anticoagulant to the Collection Bag
Perform the following steps to add the appropriate anticoagulant to the collection bag:
1
Clamp the line above the tubing containing the frangible connector on the accessory line of the collection bag.
2
Completely break the frangible connector by bending the tubing back and forth.
3
Using aseptic technique, remove the cap from the luer connector below the sterile barrier filter, and attach a
syringe containing the desired amount of anticoagulant to the connector.
4
Unclamp the line above the frangible connector.
5
Slowly inject the anticoagulant through the sterile barrier filter into the collection bag.
6
Clamp the line above the frangible connector.
7
Remove the syringe from the luer connector.
8
To ensure that you delivered all of the anticoagulant in the syringe into the collection bag, perform the
following steps:
a. Attach a syringe containing at least 2.3 mL of saline to the luer connector. (The volume of the accessory
line and sterile barrier filter is approximately 2.3 mL.)
b. Unclamp the line above the frangible connector.
c. Slowly inject the saline through the sterile barrier filter to flush the anticoagulant from the filter into the
collection bag.
d. Clamp the line above the frangible connector.
e. Remove the syringe from the luer connector.
Note:
To prevent fluid from flowing from the collection bag towards the luer connector, do not remove the syringe
from the luer connector before you clamp the line above the frangible connector.
SpectraOptiaOpsManual.book Page 270 Thursday, May 17, 2018 11:24 AM
