White Blood Cell Depletion (WBCD) and Platelet Depletion (PLTD) Procedures
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Spectra Optia Apheresis System Operator’s Manual
Functional Description of a White Blood Cell Depletion (WBCD) or a
Platelet Depletion (PLTD) Procedure
During a WBCD or a PLTD procedure, the system pumps the patient’s blood into the tubing set and spins the
centrifuge at the speed required to target the optimal (default) packing factor: 4.5 for a WBCD procedure using
ACD-A, and 20 for a PLTD procedure. The AIM system adjusts the flow rate of the plasma pump to control the
concentration of cells that flow through the collect port (based on the collection preference). When the AIM
system detects cells in the collect port, the system sounds a tone, the collect valve moves into the collect position,
and the collect pump pumps the cells into the collection bag. The plasma pump pumps plasma out of the channel
and into the reservoir. The red blood cells (RBC) are pushed out of the channel and into the reservoir, where they
combine with the plasma for return to the patient.
If the operator chooses to give replacement fluid during the run, the plasma valve periodically moves to the return
position to allow small volumes of replacement fluid to flow by gravity into the reservoir. In the reservoir, the
replacement fluid combines with the RBC and the plasma from the channel. The system manages the volume of
cells collected and fluid replaced according to the fluid balance the operator selects.
Preparing to Perform a Procedure
Supplies
Assemble the supplies listed on page 62 before you begin a procedure. You will also need the following additional
supplies:
•
Replacement fluid prescribed by the patient’s physician
Anticoagulant Solutions for WBCD Procedures
You can use ACD-A or a solution that contains hydroxyethyl starch (HES) to perform a WBCD procedure on the
Spectra Optia system.
Using ACD-A
To indicate the use of ACD-A, you must select
No
for HES on the WBCD configuration screen or on the options
screen. “HES: No” appears on the main run screen as a reminder of your selection. The default packing factor when
ACD-A is used is 4.5.
Using HES
To indicate the use of HES, you must select
Yes
for HES on the WBCD configuration screen or on the options
screen. “HES: Yes” appears on the main run screen as a reminder of your selection.When using HES, you must add
trisodium citrate to the HES for use as the anticoagulant. The default packing factor when HES is used is 1.6.
Preparing an anticoagulant solution containing HES and trisodium citrate
To prepare the correct anticoagulant solution, add 30 mL to 40 mL of 46.7% trisodium citrate solution to 500 mL
of HES. To prepare an anticoagulant solution with the same concentration as ACD-A, add 38 mL of trisodium
citrate solution to the HES.
Note:
Using a filter for the replacement fluid during a WBCD or a PLTD procedure is not recommended. A filter can
slow the flow of the replacement fluid into the reservoir and ultimately to the patient.
SpectraOptiaOpsManual.book Page 178 Thursday, May 17, 2018 11:24 AM
