Mononuclear Cell (MNC) Collection Procedures
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Spectra Optia Apheresis System Operator’s Manual
Functional Description of a Mononuclear Cell (MNC) Collection
Procedure
The Spectra Optia system cycles through a number of accumulation and collection phases during an MNC
collection procedure. The number of phases depends on the patient’s WBC count and the goals of the procedure.
Accumulation phase
During the accumulation phase, the system pumps the patient’s blood into the tubing set and spins the centrifuge
at the speed required to target the optimal (default) packing factor of 20. The AIM system adjusts the plasma pump
flow rate to control the concentration of cells that flow through the collect port (based on the collection
preference), and the collect pump pumps the MNC and platelets from the connector into the chamber. The
chamber fills with MNC and the platelets flow through the chamber and are returned to the patient.
The plasma pump also pumps plasma out of the channel and either into the reservoir, or into the plasma bag or the
collection bag if plasma collection is targeted. The red blood cells (RBC) are pushed out of the channel and into the
reservoir, where they combine with the plasma for return to the patient.
Collection phase
A collection phase occurs at the beginning of the second and all subsequent accumulation phases. The collection
phase begins once the system predicts that the chamber is at least 50% full of MNC, and the RBC detector detects
RBC in the collect line. When the phase begins, the system stops the plasma pump to lower the interface, stops the
collect pump, and decreases the centrifuge speed. It then restarts the collect pump and moves the collect valve into
the collect position. The collect pump pumps plasma through the chamber to flush the chamber contents into the
collection bag.
After the system flushes the chamber, it stops the collect pump and increases the centrifuge speed. It then restarts
the plasma pump and the collect pump to start the chase. During the chase, the system displaces the cells remaining
in the collect line between the chamber and the collect valve into the collection bag before it moves the collect valve
into the return position to end the collection phase.
Preparing to Perform a Procedure
Supplies
Assemble the supplies listed on page 62 before you begin a procedure.
Selecting the Procedure and Loading the Tubing Set
Follow the instructions starting on page 64 to select the procedure and load the tubing set. Before you load the
tubing set, verify that the set and the solutions you are using have not expired by checking the expiration date
printed on the packaging. Do not use an item if the current date is past the expiration date.
SpectraOptiaOpsManual.book Page 132 Thursday, May 17, 2018 11:24 AM
