SF-8200 Operation Manual
8
/
76
liquid is platelet poor plasma (PPP).
3) Sample placement
Coagulation Test The PT test sample should be tested within 24 hours at (18-24) °C; the APTT test
sample should be tested within 4 hours; other tests (eg FIB, PC, Factor V, Foctor VIII, D-Dimer, FDP, etc. )
Should be tested within 4 hours.
If the clotting test cannot be performed immediately, the sample can be stored at 4 °C for 2 hours. If
the PT test sample cannot be tested within 24 hours or the APTT and other tests cannot be tested within
4 hours, the plasma sample can be stored frozen below-20 °C for two weeks and below -70 °C for 6
months. Frozen samples should be rapidly thawed at 37 °C and tested immediately. Frozen samples may
affect the APTT test.
4.2 PREPARATION OF REAGENTS
1) Use of reagents
Users in the reagent before use, be sure to read the instructions inside the kit, and strictly in ac-
cordance with the provisions of the manual to use and save.
Test reagent configuration and dosage can refer to the user to use the reagent packaging and in-
structions, reagents should choose a legal solidification method, the optical method of reagent determi-
nation of the box, the use of different batches and manufacturers of reagents need to do calibration,
coagulation test for calibration Calibrators must be used, different batches, different components cannot
be mixed.
2) PT reagent selection
Select a reagent labeled ISI (International Sensitive Index) for quality control. The lower the ISI
value of the reagent, the more sensitive it is to each coagulation factor measured.
4.3 PROJECT SETTINGS AND INSTRUMENT STATUS
1) Project Settings
Experimental settings must be provided by the manufacturer of reagents set, such as temperature
incubation time, test time, and sample, reagent, buffer, the amount of cleaning fluid.
2) Needle and pipeline maintenance
The purpose of the needle maintenance is to clean the outer surface of the needle to avoid
cross-contamination affect the test results. The purpose of pipeline perfusion maintenance is to make the
pipe filling part of the filling system full of cleaning liquid to reduce the wall bubble, to avoid the amount of
sample is not allowed to affect the test results.
Every morning before the start of the test need to carry out the needle and the pipeline maintenance,
in the course of one day of use, half an hour to suspend the use of the instrument, please use pipeline
maintenance before use.