Reichert Univar, User Manual

Page: 31 / 73

C3
Conventional fluorescence and quinacrine with
epifluorescence
With the epifluorescence illuminator, you can
simultaneously perform all illumination methods
using the interference beamsplitter mirror 40. For
isolation of paler details we recommend transmitted-
light phase contrast (page C9) or transmitted-light
darkfield (page C7) to sharpen the specimen image
Conventional fluorescence and quinacrine:
⚪ Turn on the HBO 200W mercury-vapor pressure
lamp
⚪ Turn off the transmitted-light halogen lamp
⚪ Turn off the neutral density filters in the body
(black dot)
⚪ Turn on relay (red dot). For weak fluorescent
objects, it can be switched off
⚪ Turn off the Bertrand lens
⚪ Turn off the main IC prism and analyzer
⚫ Put on the UV-blocking orange shade
⚫ Rotate into place the 10x objective
⚫ Slide the interference mirror switch to 40 or 49,
depending on the desired excitation (page C4)
⚪ Set the photo system magnification changer to
low magnification (red dot, L)
⚪ Direct beam path to the camera (red dot, CAM)
⚪ Set beam to the body to 20% (red dot,CAM/PRO)
⚪ For weak fluorescent preparations, set to EYE
⚪ Switch off the phase ring knob (red dot)
⚪ Set the relay magnification changer to 1x (red
dot)
⚫ Set the swivel mirror to high-performance lamp
⚫ Set the rotary prism to high-performance lamp
⚪ Sharpen with coarse and fine focus on the
fluorescent image
⚫ Adjust the high-performance light collector to
evenly illuminate the field
⚪ Adjust the field iris diaphragm opening in the
epifluorescence illumination pipe (page C4) with the
knurled wheel; to focus the iris image, loosen the
hex pin and move axially. Tighten the pin and open
the field iris diaphragm
NOTE:
The filters in the epifluorescence attachment have a
colored label for each fluorescence method. These
fluorescence methods can also be done with
transmitted-light exciter using the corresponding
transmission exciter filter with the same color, on
the mirror house 2 or 4