Reichert Univar, User Manual

Page: 23 / 73

B13
Transmitted-light darkfield fluorescence:
⚪ Turn on HBO 200 W mercury vapor high-pressure lamp
⚪ Turn on relay (red dot). For weak fluorescent objects, it can
be switched off
⚪ Pull out pin of Bertrand lens (set off)
⚪ Turn off neutral density filters in the ocular body (black dot
not visible)
⚫ Slide the UV blocking filter slide to the white marked
blocking filter
⚫ Mount the UV blocking lamp shade
⚪ Rotate the compensator out of position
⚪ Pull out the interference contrast (IC) main prism to the
stop, after loosening the clamping screw on the left side
⚫ Use the 40x objective when using the immersion darkfield
condenser; when working with the dry darkfield condenser,
use the 25x objective
⚫ Rotate into place the darkfield condenser, and oil with
fluorescence-free immersion oil (Cargille D/A)
⚪ When working with the dry darkfield condenser, with apo
25x and plan 40x objectives, the aperture insert is inserted
into the condenser
⚪ Swing out the transmitted-light filter polarizer
⚪ Turn the lower condenser turret to the empty opening (red
dot)
⚫ Set condenser fine drive in the central position and raise
the condenser with the coarse drive up to the stop, with liquid
on the condenser top to form a uniform liquid contact between
the specimen slide and the immersion condenser
⚫ Put UV shade tube on light well
⚪ Set the collector of the high-performance light on the mark
⚪ Switch off the auxiliary optics for wide field condenser (red
dot)
⚪ Set off exciter filters for transmitted-light (red dot)
⚪ Set off neutral density filters for transmitted-light (red dot)
⚪ Adjust the photo system magnification changer to low
magnification (red dot, L)
⚪ Direct the beam path to the camera (red dot, CAM)
⚫ Direct the beam (20%) to the body (red dot, CAM/PRO).
With weak fluorescent preparations, use position EYE
⚪ Switch off the phase ring knob (red dot)
⚪ Set the relay magnification changer to 1x (red dot)
⚫ Set the rotary prism for transmitted-light with high-
performance lamp
⚪ Set off the color filters for contrast fluorescence (red dot)
⚫ For darkfield UV exciter filter use (mirror house 4) exciter
filter U2; (mirror house 2) exciter filter S2 (white)
⚫ Set sliding mirror for high-performance lamp
⚫ Switch off the automatic zoom lighting (lever to the side)
⚫ Set manual zoom lighting to DF
⚫ Fully open condenser iris diaphragm
⚪ With coarse and fine adjustment, focus on a specimen
⚫ Close the field iris aperture and focus on the diaphragm
image with the condenser fine adjustment
⚫ Center the image of the iris diaphragm, with centering
screws on the condenser rack. Then open the field iris
diaphragm just larger than the field of view
⚪ Modulate glare from strongly fluorescent specimens with
the objective iris (if present)