Reichert Univar, User Manual

Page: 34 / 73

C6
Field iris diaphragm aperture illumination for
microphotometry
There is an iris diaphragm in the epifluorescence
illuminator light pipe. This is adjusted to prevent UV
fading of large areas of the specimen. Opening the
field iris diaphragm is done by rotating the knurled
ring, while focusing on the iris is done by loosening
the hex lock pin and using it to slide the cylindrical
part towards or away from the observer, then
retightening the pin.
In the light pipe is an opening for inserting an
aperture slide with a hole of desired size for
microphotometry. These apertures are used when
measuring the smallest object details, and are
centered and focusable.
Light filter in the epifluorescence illuminator for aromatic amines
Exciter filter, interference divider mirror and blocking filter are coupled to each other and can be adjusted at the
same time with the help of the sliding lever located on the front of the attachment.
The number designation is derived from the long wavelength end of the reflection band of the individual
interference beam-splitting mirror, in nm divided by 10 (nm/10).
Filter
Marking
Exciter
filter
Interference mirror
Blocking
filter
Use
40
White
UV exciter filter 18x2
UG 1
UV reflecting up to
approx. 400nm
18x3 KV 418/VG
For UV-excitation,
dopamine and
derivatives
45
White
Violet exciter filter
18x5, λ=405nm
UV + blue reflecting
up to approx. 450 nm
18x3 KV 418/VG
For Violet excitation,
adrenaline,
noradrenaline,
serotonin.
Narrow band exciter
with very good
distinction
45°
yellow
Violet exciter filter
18x2, λ=425nm
UV + Blue reflective
up to approx. 450 nm
18x3 (GG 9 + G2 +
GG 9)/VG
For Violet excitation,
adrenaline,
noradrenaline,
serotonin.
Brighter fluorescence
than above, however
less distinction
56
Green
Cover plate
Green reflective until
approx. 560 nm
18x3 OG 590/VG
By default, no
function.
The built-in filters are
intended for future
expansion