Reichert Univar, User Manual

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B9
Transmitted-Light Interference Contrast
(A) with halogen lamp
⚪ Turn on halogen lamp power supply
⚪ Turn on relay (red dot)
⚪ Disengage Bertrand lens (pull out pin)
⚪ Turn off neutral density filters in the ocular body (no
black dot)
⚪ Remove blocking-filter slide or rotating analyzer (the
white marked blocking-filter can be left in the light path)
⚪ Remove the compensator
⚫ Slide the interference contrast main prism up to the stop
and clamp with the screw located on the left side
⚫ Rotate in the 10x objective; interference contrast studies
can be carried out with this magnification. Only IK low-
power lenses may be used
⚪ Remove exciter or color filter for transmitted-light (red
dot)
⚪ Engage neutral density filters for transmitted-light
⚫ Rotate to brightfield condenser on upper turret; the
following strain-free condensers (P) can be used: dry
condenser, NA=0.90; immersion condenser, NA=1.30
⚪ Swing out the polarizer filter
⚫ On lower condenser turret, rotate to the compensation
prism 10 IC
⚫ Set condenser fine feed in the central position and raise
condenser with coarse feed to the stop
⚪ Switch off the auxiliary optics for wide-field condenser
(red dot)
⚪ Adjust collector for halogen lamp (red dot)
⚪ Adjust the photo system magnification changer to low
magnification (red dot, L)
⚪ Redirect beam path to the camera (red dot, CAM)
⚪ Set ocular body light to 20% (red dot, CAM/PRO)
⚪ Switch off the phase ring knob (red dot)
⚪ Set the relay magnification changer to 1x (red dot)
⚪ For mirror house 2, set rotary prism for transmitted-light
with halogen lamp
⚪ Switch off color filters for contrast fluorescence (red dot)
⚪ On mirror house 2, set excitation filter to red dot
⚪ Set sliding mirror for halogen lamp (red dot)
⚪ Turn on automatic zoom lighting (red dot)
⚪ Focus on a specimen with coarse and fine adjustment
⚫ Close down field iris aperture and focus on the image
with condenser fine feed
⚫ Center field iris diaphragm with centering screws; then
open field iris diaphragm just larger than the field-of-view
⚫ Close condenser iris diaphragm so far that the
microscopic image appears clear and full of contrast
⚫ Adjust the interference contrast main prism by turning
the knurled screw, until the desired contrast, black and
white or colored, appears
⚫ When moving to stronger objectives, the similarly
marked IC prism is rotated in with the condenser hub