Reichert Univar, User Manual

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C4
Field iris diaphragm & aperture slot for microphotometry
There is an iris diaphragm in the epifluorescence illuminator light pipe.
This is adjusted to prevent UV fading of large areas of the specimen.
Opening the field iris diaphragm is done by rotating the knurled ring,
while focusing is done by loosening the hex lock pin and using it to
slide the cylindrical part towards or away from the observer, then
retightening the pin.
In the light pipe is an opening for inserting an aperture slide with a hole
of desired size for microphotometry. These apertures are used when
measuring the smallest object details and are centered and focusable.
Filter
Number
Exciter filter Interference mirror Blocking filter Use
40
(White)
UV exciter
filter 18x2 UG 1
UV reflecting up to approx.
400nm
18x3 KV 418/VG
For UV stimulation, primary
fluorescence, secondary
fluorescence
45
Yellow
Cover plate
UV-blue reflecting up to
approx. 450nm
18x3 (GG 9 + G2 +
GG 9)/VG
By default, no function.
The built-in filters are
intended for future
expansion
49
Blue
Blue exciter
filter 18x3 BG 12
Blue reflective until approx.
490nm
18x3 (GG 9 +
OG 515)/VG
Blue light fluorescence,
quinacrine, primary
fluorescence and secondary
fluorescence with commonly
used fluorochromes. The
filters enable the excitation
of FITC with the very strong
436nm emission line of the
Hg lamp. These are the
default filters for blue
excitation.
56
Green
Cover plate
Green reflective until
approximately 560nm
18x3 OG 590/VG
By default, no function.
The built-in filters are
intended for future
expansion
Light filter in the epifluorescence illuminator for conventional fluorescence and quinacrine
Exciter filter, interference divider mirror and blocking filter are coupled to each other and can be adjusted at the
same time with the help of the sliding lever located on the front of the house.
The number designation is derived from the long wavelength end of the reflection band of the individual
interference beamsplitting mirror, in nm divided by 10 (nm/10).