Reichert Univar, User Manual

Page: 22 / 73

B12
Fluorescence immersion condenser
N.A.: 1.35 oil
Free working distance: 0.37mm
Use: For fluorescence microscopy in brightfield, with
objectives between 10x and 100x, to meet the highest
requirements of brightness and contrast.
Also for working with normal light with 4x objective
(use the frosted 4 filter on the lower condenser turret)
Brightfield immersion condenser
N.A.: 1.30 oil
Free working distance: 0.42mm
Use: this condenser can be used in normal situations
for contrast fluorescence and fluorescence microscopy.
Only a small brightening of the image background will
occur
You can perform fluorescent work with objectives of
10x to 100x. Also for working with normal light with 4x
objective (use the frosted 4 filter on the lower
condenser turret)
Blocking filter slide for fluorescence
The blocking filter slide is inserted into the
transmitted-light optics carrier. The spring retainer on
the front of the optics carrier is pulled out and the
empty slide removed. The colors of the notch filters
must be visible from the front.
Light shade
The light shade protects the observer from stray UV
light. It is magnetically attached underneath the ocular
body of the microscope stand.
UV light blocking tube
The light tube is placed on the light well of the
microscope base and protects from UV glare. In
addition, it can be used to hold various light filters with
a 50mm diameter.
For an overview of the use and combination of the
excitation and blocking filters, please read the
fluorescence methods descriptions of the mirror
houses 2 and 4.