Reichert Univar, User Manual

Page: 37 / 73

C9
Mixed illumination: epifluore transmitted-light
phase- and anoptral-contrast
(A) Transmitted-light phase- and anoptral-contrast, halogen
lamp
⚪ Turn on halogen lamp. Turn on relay (red dot)
⚪ Turn off Bertrand lens (pull out pin)
⚪ Turn off neutral density filters in ocular body (no black dot)
⚪ Turn off the analyzer or interference-contrast prism
⚫ Turn on interference beamsplitting mirror 40
⚫ Engage the 10x objective (all transmitted-light objectives from
10x up are suitable for phase- or anoptral-contrast)
⚪ Switch off transmitted-light exciter filters (red dot)
⚪ Switch off transmitted-light neutral density filters (red dot)
⚫ Turn to brightfield condenser (following condensers can be used:
immersion condenser NA=1.30 and dry condenser NA=0.90)
⚪ Swing out transmitted-light filter polarizer
⚫ Rotate the lower condenser turret to 10 Ph
⚫ Set condenser fine drive in the central position and lift
condenser with coarse feed up to the stop
⚪ Switch off the auxiliary optics for wide field condenser (red dot)
⚪ Set collector for transmitted-light halogen lamp (red dot)
⚪ Adjust the photo system magnification changer to low
magnification (red dot, L)
⚪ Direct the beam path to the camera (red dot, CAM)
⚪ Direct beam (20%) to the ocular body (red dot, CAM/PRO)
⚫ Rotate the phase ring per the objective: e.g., 10 for phase
contrast or 10A for anoptral-contrast, for 10x objective
⚪ Set magnification changer to 1x (red dot)
⚫ On mirror house 2, turn rotary prism for transmitted-light using
the halogen lamp
⚪ Switch off the color filters for contrast fluorescence (red dot)
⚪ On the mirror house 2, set exciter filter to red dot
⚪ Set the sliding mirror to the halogen lamp (red dot)
⚪ Turn on automatic zoom lighting (red dot)
⚪ Focus on a specimen with coarse and fine adjustment
⚫ Close field iris diaphragm, then focus the its image in the
specimen image plane with condenser fine focus
⚫ Center field iris diaphragm in the middle of the field with
centering screws on the condenser. Then open field iris diaphragm
just wider than the field of view.
⚫ Open the condenser iris wide open
⚪ When using stronger objectives, don't forget to use the
appropriate contrast ring setting