Reichert Univar, User Manual

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B11
Transmitted-light brightfield fluorescence:
⚪ Turn on HBO 200W mercury-vapor high-pressure lamp
⚪ Set light path to relay (red dot). With weak fluorescent
objects it can switched off
⚪ Disengage Bertrand lens by pulling out pin
⚪ Disengage neutral density filters in the ocular body (black
dot not visible)
⚫ Slide the UV blocking filter slide to the yellow-marked filter
⚫ Mount the UV blocking eye shade
⚪ Rotate the IC prism out of position
⚪ Pull out the main (upper) interference contrast prism until it
stops, after loosening the clamping screw on the left side
⚪ Rotate the 10x objective into position
⚫ Set the collector knob of the respective high-performance
light to the mark
⚫ Position the fluorescence immersion condenser and oil with
fluorescence-free immersion oil (Cargille D/A)
⚪ Swing out the transmitted-light filter polarizer
⚫ Set condenser fine drive in the central position and raise
condenser up to the stop with the coarse drive. A uniform liquid
contact must be formed between the specimen slide and the
condenser
⚪ Move the condenser turret to the empty opening (red dot)
⚫ Set UV light-shield tube over the light outlet
⚪ Switch off the exciter filter for transmitted-light (red dot)
⚪ Switch off the neutral density filters for transmitted-light (red
dot)
⚪ Switch off the auxiliary optics for wide-field condenser (red
dot)
⚪ Adjust the photo magnification changer to low (red dot, L)
⚪ Direct the beam path to the camera (red dot, CAM)
⚫ Set the beam to 20% to the ocular body (red dot, CAM/PRO)
For weak fluorescent preparations, use EYE position
⚪ Set the phase ring knob to its empty setting (red dot)
⚪ Set the relay magnification changer to 1x (red dot)
⚫ Set the rotary prism to the high-performance lamp position
⚪ Set off the color filters for contrast fluorescence (red dot)
⚫ Turn on exciter filter for UV brightfield
⚪ Mirror house 4: exciter filter U1. Mirror house 2: filter S1
⚫ Set sliding mirror for high-performance lamp
⚪ Turn on automatic zoom lighting (red dot)
⚪ Turn on the power for the zoom lighting with the halogen
lamp power supply
⚫ Fully open condenser iris diaphragm
⚪ Focus on a specimen with coarse and fine adjustment
⚫ Slightly close field iris aperture and focus on the iris image
with condenser fine adjustment
⚫ Center the field iris diaphragm, then open the field iris
diaphragm just larger than the field of view
⚪ The glycerin immersion objectives are particularly suitable
for higher magnifications. With strongly fluorescent
preparations, the objective iris can be closed down to control
glare