Reichert Univar, User Manual

Page: 35 / 73

C7
Mixed illumination: epifluore transmitted-
light darkfield, epifluore transmitted-light
darkfield fluorescence
A) transmitted-light darkfield with halogen lamp:
⚪ Turn on halogen lamp with control transformer
⚪ Turn on relay (red dot)
⚪ Turn off neutral density filters in the ocular body (dot not
visible)
⚪ Turn off Bertrand lens (pull out pin)
⚫ Set interference beamsplitter mirror to 40
⚫ Turn to 40x objective when using the immersion darkfield
condenser. Turn to 25x objective when working with the dry
darkfield condenser located on the condenser turret. Use
the 10x objective if the condenser is on the quick coupler
⚪ Turn off exciter or filter for transmitted-light (red dot)
⚪ Turn off neutral density filters for transmitted-light (red
dot)
⚫ Turn to darkfield condenser. Apply immersion oil to the
immersion darkfield condenser. When working with the dry
darkfield condenser and 40x or 25x apo objectives, an
aperture is inserted into the condenser
⚪ Swing out the transmitted-light filter polarizer
⚫ Set condenser fine focus in the central position and use
the coarse focus to raise condenser up to the stop. A
uniform liquid contact should occur between the immersion
darkfield condenser and the specimen slide
⚪ Turn the lower condenser turret to the empty opening
(red dot)
⚪ Rotate off the auxiliary optics for wide field condenser
(red dot)
⚪ Adjust the collector for the transmitted-light halogen
lamp (red dot)
⚪ Adjust the photo system magnification changer to low
magnification (red dot, L)
⚪ Direct beam path to the camera (red dot, CAM)
⚪ Direct 20% beam to the ocular tube (red dot, CAM/PRO)
⚪ Rotate the phase ring knob to the empty position (red
dot)
⚪ Set relay magnification to 1x (red dot)
⚪ On mirror house 2, set the rotary prism to transmitted-
light with halogen lamp
⚪ Turn off the analyzer or interference contrast main prism
⚪ On mirror house 2, set exciter filter to red dot
⚪ Switch off color filters for contrast fluorescence (red dot)
⚪ Set the sliding mirror to halogen lamp (red dot)
⚫ Switch off the automatic zoom lighting (lever to the side)
⚫ Set manual zoom lighting to DF
⚫ Fully open condenser turret iris diaphragm
⚪ Focus on a specimen with coarse and fine adjustment
⚫ Slightly close field iris and focus on its image with
condenser fine adjustment
⚫ Center field iris diaphragm in the middle of the field with
centering screws on the condenser. Then open field iris
diaphragm just wider than the field of view