Reichert Univar, User Manual

Page: 42 / 73

C14
B) Epifluorescence
⚪ Turn on the HBO 200W mercury vapor lamp
⚪ Turn off the halogen lamp
⚪ Mount the UV blocking shade
⚫ For the desired UV exciter, set the interference
beam-splitting mirror from 40 to 56 for
immunofluorescence (page C1), aromatic amines
(page C5), or conventional fluorescence (page C3)
⚫ Rotate the swivel mirror for light from the HBO
lamp
⚫ Turn the rotary prism for light from the HBO lamp
⚪ With immunofluorescence, swing out the light-
pipe filter opaque plate, and if necessary swivel into
place additional color filters and open the electronic
shutter
⚪ Adjust the sharpness of the fluorescent image
with fine adjustment
⚫ Evenly light the field with the collector of the
high-performance lamp
⚫ With weak fluorescent images, divert 100% light
to the oculars (EYE). You may also switch off the
relay
⚪ Adjust the field iris diaphragm (in the
epifluorescence light-pipe) using the knurled ring,
and sharpen the iris image in the image plane by
loosening hex post and sliding fore-and-aft
C) Mixed illumination
⚪ Turn on the halogen lamp so that the
epifluorescence image and the transmitted-light
interference contrast image can be seen at the
same time
⚪ To better distinguish between of the two images,
you can rotate in a contrast filter for the halogen
lamp
Transmitted-light analyzer in the
epifluorescence attachment
The analyzer is built into a slide and oriented North-
South. It can be inserted into the microscope after
pulling out the spring retainer which is located in
front of the epifluorescence head
Two stop positions is as follows:
Push the slide to the left stop position = Analyzer off
Push the slide to the right stop = Analyzer on
Transmitted-light polarizer
The swing-out polarizer is mounted on the underside
of the condenser. The east-west plane of polarized
light is the working position.