Reichert Univar, User Manual

Page: 33 / 73

C5
Detection of aromatic amines with
epifluorescence
With the epifluorescence illuminator, you can
simultaneously perform all light methods using the
interference beamsplitter mirror 40. For isolation of
paler details, we recommend transmitted-light
phase contrast (page C9) or transmitted-light
darkfield (page C7) to sharpen the specimen image.
Verification of aromatic amines
⚪ Turn on HBO 200W mercury vapor high-pressure
lamp
⚪ Turn off transmitted-light halogen lamp
⚪ Turn off the neutral density filters in the body
(black dot)
⚪ Turn on relay (red dot). For weak fluorescent
objects, it can be switched off.
⚪ Turn off the Bertrand lens
⚪ Turn off the main IC prism and analyzer
⚫ Put on the UV-blocking orange shade
⚫ Rotate into place the 10x objective
⚫ Slide the interference mirror switch to 40, 45 or
45°, depending on the desired exciter (page C6)
⚪ Set the photo system magnification changer to
low magnification (red dot, L)
⚪ Direct beam path to the camera (red dot, CAM)
⚪ Set beam to 20% (red dot,CAM/PRO)
⚪ For weak fluorescent preparations, set to to EYE
⚪ Switch off the phase ring knob (red dot)
⚪ Set the relay magnification changer to 1x set (red
dot)
⚫ Set the swivel mirror to high-performance lamp
⚫ Set the rotary prism to high-performance lamp
⚪ Focus with coarse and fine adjustment on the
fluorescence
⚫ Adjust the high-performance light collector to
evenly illuminate the field
⚪ Adjust the field iris diaphragm opening in the
epifluorescence illumination pipe (page C4) with the
knurled wheel; to focus the iris image, loosen the
hex pin and push or pull. Tighten the pin. Open the
field iris diaphragm.
NOTE:
The filters in the epifluorescence attachment have a
special colored icon for each fluorescence method.
These fluorescence methods can also be done with
transmitted-light excitation using the corresponding
transmissive exciter filter with the same color on the
mirror house 2 or 4.