Reichert Univar, User Manual

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C1
Immunofluorescence with epifluorescence attachment
With the epifluorescence optics carrier, the interference splitter
mirror set on 40 can perform all transmitted-light and
simultaneous methods. To achieve best specimen detail we
recommend transmitted-light phase contrast (page C9) or
transmitted-light darkfield (page C7).
Immunofluorescence:
⚪ Turn on HBO 200W mercury vapor high-pressure lamp
⚪ Turn off transmitted-light halogen lamp
⚪ Turn off neutral density filters in the body (black dot not
visible)
⚪ Turn on relay (red dot); with weak fluorescent objects, it can
be switched off
⚪ Turn off Bertrand lens (pull out pin)
⚪ Turn off the main interference contrast prism and analyzer
⚫ Mount the UV blocking shade
⚫ Switch to 10x objective (if not already using transmitted-light
phase contrast or darkfield on the specimen)
⚫ Set the interference beam-splitting mirror slide to 40, 49,
49°, or 56, depending on the desired excitation (page C2)
⚪ Adjust the photo magnification changer to low (red dot, L)
⚪ Direct beam path to the camera (red dot, CAM)
⚪ Direct 20% beam to the body (red dot, CAM/PRO)
⚪ With weak fluorescent preparations, use EYE position
⚪ Set phase ring knob to empty position (red dot)
⚪ Set relay magnification changer to 1x (red dot)
⚫ Set the swivel mirror to high-performance lamp
⚫ Set the rotary prism to high-performance lamp
⚫ If available: pivot into place any desired color filters, and
open electronic shutter
⚪ Focus on the fluorescent image with coarse and fine
adjustment
⚫ Adjust the collector of the high-performance light to evenly
illuminate the field
⚪ With the knurled wheel, close the field iris diaphragm in the
illumination tube (page C4) and focus on your specimen image
after loosening the hex pin. Open the field iris diaphragm.
NOTE: Each filter in the epifluorescence carrier has a colored
indicator for each fluorescence method. If you can also perform
these fluorescence methods with transmitted-light excitation,
mirror houses 2 or 4 are marked with the same color on the
mirror house exciter filters