Reichert Univar, User Manual

Page: 38 / 73

C10
B) epifluorescence
⚪ Turn on HBO 200W mercury-vapor lamp
⚪ Turn off the transmitted-light halogen lamp
⚪ Mount the UV blocking shade
⚫ Switch to the desired exciter interference beam-
splitting mirror (40 to 56) - as described (page C1),
aromatic amines (page C5), or conventional
fluorescence (page C3) for immunofluorescence
⚫ Rotate the swivel mirror for epi-illumination from
the HBO lamp
⚫ Turn the rotary prism for epi-illumination from the
HBO lamp
⚪ If immunofluorescence is present, swing out the
cover plate in the light-pipe hinged filter group, and
perhaps swivel additional color filters into the light-
pipe, and open the electronic shutter. Correct the
sharpness of the fluorescence image with fine focus
⚫ Adjust the collector of the HBO lamp to evenly
illuminate the field
⚫ For weak fluorescence, divert 100% of the light to
oculars (EYE)
⚪ Close the field iris diaphragm in the
epifluorescence light-pipe with the knurled ring, and
focus your image after loosening the hex shaft and
moving forward or backward
C) mixed light:
⚪ Turn on the halogen lamp so that the
epifluorescence image and transmitted-light phase
contrast image can be seen simultaneously; to
better distinguish between the two images, you can
insert a contrast filter into the halogen lamp light
path
Phase ring knob with phase and anoptral
annuli
This turret contains the phase and anoptral annuli
(rings) that be inserted into the relay optical path.
The annuli are marked with the lens magnification of
the appropriate objective, e.g., the phase ring 10
and the anoptral ring 10A are for the 10x objective.
In the position 0 (red dot), the knob positions the
empty hole into the beam path
Ring aperture
The phase/anoptral rings are included in the lower
rotating condenser turret. For correct centering,
each ring must be adjusted to the image of the
corresponding ring diaphragm (in the relay system
turret) when changing objectives (page B6)