Nikon Eclipse TS100 User Manual Download

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培養倒立顕微鏡

ECLIPSE

TS100/TS100-F

TS100LED MV/TS100LED-F MV

使用説明書

Inverted Microscope

ECLIPSE

TS100/TS100-F

TS100LED MV/TS100LED-F MV

Instructions

M590J/E

11.10.NC.1 (1/2)

Summary of Contents for Eclipse TS100

Page 1: ...培養倒立顕微鏡 ECLIPSE TS100 TS100 F TS100LED MV TS100LED F MV 使用説明書 Inverted Microscope ECLIPSE TS100 TS100 F TS100LED MV TS100LED F MV Instructions M590J E 11 10 NC 1 1 2 ...

Page 2: ... been made to ensure the accuracy of this manual errors or inconsistencies may remain If you note any points that are unclear or incorrect please contact your nearest Nikon representative Some of the equipment described in this manual may not be included in the set you have purchased If you intend to use any other equipment with this product read the manual for that equipment too If the equipment ...

Page 3: ...y E 22 4 Operation of Each Part E 23 1 Aperture Diaphragm E 23 2 Specimen and Stage E 24 3 Focusing Device E 25 4 Filters E 26 5 Objectives E 26 6 Modulation Contrast Method E 29 7 Photomicrography for TS100 F TS100LED F MV only E 31 5 Assembly E 33 1 Assembling the Basic Set E 33 2 Assembling Components Not Included in the Basic Set E 36 6 Troubleshooting E 38 1 Optical E 38 2 Operational E 42 3 ...

Page 4: ... of the following WARNING Spillage of a sample from a vessel onto the microscope presents a biohazard risk To avoid biohazard contamination do not touch the contaminated portion with your bare hands Decontaminate the contaminated portion according to the standard procedure of your laboratory CAUTION for heat for TS100 TS100 F only This symbol label attached on the lamphouse cover reminds you of th...

Page 5: ...he instructions below if this is the case Use of the microscope under the incorrect voltage may cause over heating due to over current possibly resulting in a fire and damaging the microscope If the voltage indicated on the nameplate differs from local voltage do not turn on the power but contact your nearest Nikon representative immediately If the voltage indicated to the immediate right of the A...

Page 6: ...ower cord If the nameplate reads 100 110 120 V Be sure to use the provided power cord Use of other power cords may result in malfunction or fire Do not use the provided power cord for other purpose See Chapter 8 Specifications 2 Performance Properties for the provided power cord If the nameplate reads 220 230 240 V Be sure to use the specified power cord Use of other power cords may result in malf...

Page 7: ...nd any other parts that may become hot Keep solvents away from the product and its surroundings when turning on off the power switch or plugging unplugging the power cord Be careful not to spill the solvents 9 Hazardous sample This microscope should mainly be used for microscopic observation and micromanipulation of living cells and tissue cultures in Petri dishes microtiter plates etc When handli...

Page 8: ...th to wipe away any moisture If liquid spills on the water tray use tissue paper to absorb it from the groove on the left Then remove the water tray cover using a hexagonal wrench Wipe dry the inside if there is any moisture Check if the lamp cable connector or AC inlet on the rear panel are wet and if so wipe them dry If any liquid or foreign matter gets inside do not use the microscope but conta...

Page 9: ... result in malfunction or fire See Chapter 8 Specifications 2 Performance Properties for the specified power cord To prevent electric shock turn off the power switch press the power switch to the position before connecting or disconnecting the power cord Note that this product is classified as Class I for electric shock protection Be sure to connect it to a protective earth terminal 5 Notes on han...

Page 10: ...nside If you accidentally spill a liquid on the microscope immediately turn off the power of the microscope press the power switch to the position and unplug the power cord Take care not to touch the cord with wet hands at this time After unplugging the microscope use a dry cloth to wipe away any moisture If liquid spills on the water tray use tissue paper to absorb it from the groove on the left ...

Page 11: ...lt in molding of or condensation on the lenses loss of precision and malfunctions Install the product in a place with little dust and dirt When storing the product place a cover over the product to protect it from dust Install the product in a place with little vibration Install and store the product on a level and sturdy table or stage that can bear the weight of the product Install the product i...

Page 12: ... actions will damage the microscope 7 Objectives When observing suspended cells in the container it is necessary to set the objective closer to the container than usual in order to achieve proper focus If the objective is switched while set this close it will strike the end of the container Lower the objective before rotating the revolving nosepiece when it has been set close like this Also before...

Page 13: ... The light source for ECLIPSE TS100 TS100 F is halogen lamp and the light source for ECLIPSE TS100LED MV TS100LED F MV is white LED 3 ECLIPSE TS100LED MV TS100LED F MV is designed for AC power of 100 to 240 V AC and 50 60 Hz thus has no fuse Lamp 2 Lamphouse Stage Condenser Objective Revolving nosepiece Fuse 3 Power cord Main body 1 Eyepiece tube Eyepiece ...

Page 14: ...roscopy though its resolution is reduced Fully open the aperture diaphragm during phase contrast microscopy During bright field microscopy close the aperture diaphragm to 70 of the numerical aperture of the objective to get a sharp image with good contrast The aperture diaphragm lever works the same for NAMC condenser Fully open the aperture diaphragm during NAMC microscopy Optical path changeover...

Page 15: ...n the operator s eyes 3 Set the diopter adjustment ring to the reference position Turn the diopter adjustment ring on the right eyepiece to align the 0 line with the reference line Also align the 0 line of the left diopter adjustment ring with the reference line 4 Fully open the aperture diaphragm Move the aperture diaphragm lever on the condenser to the full right to fully open the aperture 5 Set...

Page 16: ...of the container See P 27 for details 4 Place the 10x objective in the optical path again Look through the right eyepiece with your right eye and without touching the focus knobs turn the right diopter adjustment ring to bring the specimen into focus 5 Look through the left eyepiece with your left eye and without touching the focus knobs turn the left diopter adjustment ring to bring the specimen ...

Page 17: ...o to 1 1 Phase Contrast Microscopy For modulation contrast NAMC microscopy go to 1 2 Modulation Contrast NAMC Microscopy For bright field microscopy go to 1 3 Bright field Microscopy 9 Turn off the lamp Press the power switch to the position to turn off the lamp After the lamphouse cools cover the microscope with a vinyl cover to prevent dust ...

Page 18: ...h the phase slider Insert the phase slider into the condenser When you are planning to use a Ph2 objective attach the annular diaphragm Ph2 to the slider in advance 3 Insert a GIF filter Insert a GIF filter into the filter holder and bring it into the optical path Voluntary The GIF filter will improve the contrast of the phase contrast image 4 Fully open the aperture diaphragm Move the aperture di...

Page 19: ...th a Ph code other than Ph1 place that objective and the annular diaphragm having the same Ph code as that objective in the optical path and center the annular diaphragm in the same manner described above 6 Select the desired phase contrast objective Turn the revolving nosepiece and place the phase contrast objective of the desired magnification in the optical path Turn the revolving nosepiece unt...

Page 20: ...on pillar 2 Attach the NAMC slider Insert the NAMC slider into the condenser Attach the slit diaphragm to the NAMC slider in advance if the slit diaphragms are removed from the slider See P 36 for details 3 Fully open the aperture diaphragm Move the aperture diaphragm lever on the condenser to the rightmost to fully open the aperture Always fully open the aperture diaphragm for NAMC microscopy If ...

Page 21: ...Remove the NAMC1 slit diaphragm from the optical path Then using a hexagonal screwdriver press one of the grooves in the slit diaphragm s circumference and rotate the diaphragm so that the lengthwise direction of the slit image becomes parallel to the modulator s G area Re insert the NAMC1 slit diaphragm in the optical path to check whether or not the two are parallel For fine adjustment turn the ...

Page 22: ... same NAMC code as the objective in the optical path and observe Depending on the specimen changing the direction of the contrast may make easier observation Change the direction of the specimen by rotating the container or change the direction of the modulator and slit diaphragm as necessary 7 Adjust the correction ring of the objective If the objective has a correction ring adjust the ring accor...

Page 23: ...till the background color becomes white If the viewfield becomes too bright by this adjustment add an ND filter to the filter holder 3 Switch to the desired objective Turn the revolving nosepiece to enter the desired objective in the optical path Turn the revolving nosepiece until you feel it click in place 4 Adjust the aperture diaphragm Adjust the size of the aperture diaphragm so that it is 70 ...

Page 24: ...diaphragm lever stops down the diaphragm Rightward rotation opens it Remove one eyepiece then insert the centering telescope Hold the flange of the centering telescope with one hand and rotate its eyepiece for focusing The images of the objectives exit pupil appears bright round and the aperture diaphragm become visible If the image of the aperture diaphragm is not seen stop down the aperture diap...

Page 25: ...wer the objective before rotating the revolving nosepiece when it has been set close like this 3 Using the mechanical stage For details on attaching the mechanical stage see P 37 Turning the upper knob of the mechanical stage moves the stage back and forth while turning the lower knob moves it right and left Take care not to bend the specimen holder frame as it is coupled with a thin plate 96 hole...

Page 26: ...s knob gives 0 2 mm of objective movement One tenth rotation of the coarse focus knob gives 3 8 mm of objective movement The coarse fine focus knobs provide a 7 mm upward stroke and 3 mm downward stroke from the reference position The torque of the coarse focus knob can be changed by turning the coarse torque adjustment ring located at the base of the coarse focus knob on the right Turning this ad...

Page 27: ...t though the microscope has a built in heat insulation filter Diffuser For TS100LED MV TS100LED F MV as a diffuser is inserted in the filter holder as standard only one more filter can be inserted 5 Objectives 1 Ph codes A Ph code PhL Ph1 or Ph2 is printed on each of the objectives for phase contrast microscopy Regardless of the magnification of the objective be sure to insert the annular diaphrag...

Page 28: ...e previous turn then adjust the focus In this way rotate the correction ring in the same direction if a better image is obtained or rotate it in the reverse direction if a poor image is obtained Repeat this operation to find the best point The 0 mm position of the correction ring is used for microscopy of a specimen with no cover glass We recommend that you take a note of the reading of a well vis...

Page 29: ...ightly 3 or 4 times using lens tissue dampened with absolute alcohol ethyl alcohol or methyl alcohol Items may be wiped more cleanly by making sure not to wipe with the same part of the tissue more than once A nice finish may be obtained by wiping again with dry lens tissue When using absolute alcohol always follow the instructions provided by the manufacturer Keep these flammable liquids away fro...

Page 30: ...intensity and or color they cannot see or sense the colorless transparent cells or bacteria These colorless transparent objects are called phase objects since they only change the phase of the light when the light passes through them The phase objects can be made visible by dyes but their life will be deprived In order to observe the living phase objects differential interference contrast and phas...

Page 31: ...ht passes through the half dark region of the modulator and appears half dark If the light is refracted by the phase object it passes either the dark or the bright region according to the difference in the refracted angle The light then appears dark or bright according to the region the light passed through In this way the phase object is made visible In modulation contrast microscopy the image ap...

Page 32: ...uch as a TV monitor and video printer according to the instructions given in the camera s operation manual 2 Photomicrographic equipment The following items are required in order to take photographs using 35 mm film with photomicrographic equipment Assemble in the numerical order given Do not use a large camera back on the photomicrographic equipment since the center of gravity becomes high and th...

Page 33: ...e secured by two clamp screws 2 PL lens large projection lens 3 TMS F mount adaptor 4 Nikon single lens reflex camera body Note that photometric feature of some models may not function on the TS100 F 5 Eyepiece cap for camera 6 Camera release 1 Look for the sample and make sure that illumination is even If illumination is uneven make the proper adjustments 2 Switch the optical path to the vertical...

Page 34: ...rom local voltage do not turn on the power but contact your nearest Nikon representative immediately If the voltage indicated to the immediate right of the AC inlet differs from local voltage change the input voltage setting as described below before turning on the power The following values may be set for the input voltage If the nameplate reads 100 110 120 V the input voltage may be set to 100 1...

Page 35: ... setting according to instructions on P 33 5 Re attach the fuse holder in its original location 3 Attaching replacing a lamp for TS100 TS100 F only In order to prevent burn injury and or electrical shock when replacing a lamp be sure to turn off the power press the power switch to the position unplug the power cord and allow the lamp and its surrounding areas to cool sufficiently before opening th...

Page 36: ...e upwards when attaching it to the filter holder 8 Connecting the power cord Be sure to use the provided or specified power cord which matches the power supply voltage in your local area Use of other power cords may result in malfunction or fire For TS100 TS100 F 1 Turn off the power of the microscope press the power switch to the position 2 Check the input voltage indication on the rear of the mi...

Page 37: ...basic set For details on the centering telescope please see the description given in Chapter 3 Microscopy Insert the annular diaphragm Ph2 to the slider if you are planning to use an objective having a Ph2 indication Insert the slider with annular diaphragm attached into the condenser slider attachment hole 2 Components for NAMC microscopy An NAMC condenser objectives for NAMC NAMC slider slit dia...

Page 38: ... shipping clamp for three turns 4 Place the mechanical stage on top of the microscope stage and secure from the bottom of the stage using two hexagonal socket head bolts 5 Remove the shipping clamp from the mechanical stage 4 Sub stages Sub stages are secured to the microscope s stage using the screw holes for attaching a mechanical stage Shipping clamp Bolts fixing the shipping clamp Securing tap...

Page 39: ...iece is turned 1 to 4 1 The specimen is dirty if dirt or dust moves when specimen is moved on stage Clean the specimen 2 Objective is dirty if dirt or dust disappears when the objective is switched Clean the objective P 46 3 Condenser lens is dirty Clean the condenser P 46 4 Aperture diaphragm is too closed Open the aperture diaphragm to an appropriate size P 23 Dirt or dust is displayed on the mo...

Page 40: ... image is not adjusted to be contained in G area of modulator Adjust the position of the slit diaphragm P 20 Condenser position is wrong Check for proper attachment to illumination pillar A specimen of culture container bottom thickness slide glass thickness or cover glass thickness specified on the objective is not used Or the specimen is upside down Place a culture container slide glass thicknes...

Page 41: ...ngeover knob to BINO position P 13 The lamp has reached the end of its product life for TS100 TS 100F Replace the lamp P 34 Input voltage is wrong Set voltage selector to match the local voltage P 33 Image is yellowish or very bluish for TS100 TS100 F only Causes Corrective Measures An NCB11 filter is not inserted Insert the NCB11 filter P 22 Lamp voltage is too low or too high Turn the brightness...

Page 42: ...ffuser into the filter holder P 15 Filter is not inserted properly Insert the filter correctly P 17 Unwanted filter is in the optical path Remove all unwanted filters from the optical path Optical path changeover knob position is wrong for TS100 F TS100LED F MV only Select optical path to be used P 13 Image dark on one side One side of the viewfield up down right or left is not focused Causes Corr...

Page 43: ... Use a culture container of thickness 2 mm or less A focal deviation is high when switching over objectives Causes Corrective Measures Diopter adjustment has not been performed Perform diopter adjustment P 15 An objective is attached incorrectly Screw the objective all the way in P 35 Binocular images not integrated Causes Corrective Measures Interpupillary adjustment has not been performed Perfor...

Page 44: ...ent P 14 Diopter adjustment has not been performed Perform diopter adjustment P 15 Brightness is inadequate Turn the brightness adjuster to attain a suitable brightness P 14 Loses focus over time Causes Corrective Measures Torque of coarse focus knob is too loose Turn coarse torque adjustment ring to tighten torque so the revolving nosepiece does not fall on its own weight P 25 ...

Page 45: ...the specified lamp P 34 Fuses are blown for TS100 TS100 F only Replace the fuses Use only the specified type P 34 Lamp cord is disconnected Connect to connector on rear of microscope P 34 Lamp flickers Brightness is unstable Causes Corrective Measures Lamp is about to burn out for TS100 TS100 F only Replace the lamp P 34 Bad connection Secure all connections Fuse holder is not attached securely fo...

Page 46: ...Electrical E 45 Lamps burn out quickly for TS100 TS100 F only Causes Corrective Measures Not using the specified lamp Use the specified lamp P 34 Input voltage is wrong Set voltage selector to match the local voltage P 33 ...

Page 47: ...olvent may result in discoloration of the plastic parts 3 Disinfecting the Microscope We recommend that you use 70 medical alcohol for normal disinfection of the microscope In case of spillage of a sample onto the microscope determine whether the sample is hazardous If the sample is hazardous follow the standard procedure of your laboratory Using organic solvent may result in discoloration of the ...

Page 48: ...logy physiology pharmacology neurology cellular biology and molecular biology Intended user It is intended for the medical professional and those who work on experimentations in the field of genetics immunology physiology pharmacology neurology cellular biology and molecular biology 2 Performance Properties 1 Model name ECLIPSE TS100 ECLIPSE TS100 F ECLIPSE TS100LED MV ECLIPSE TS100LED F MV 2 Opti...

Page 49: ... attachment 12 Illumination light source TS100 TS100 F Lamp Rating 6 V 30 W halogen lamp PHILIPS 5761 Average lamp lifetime 100hrs Output Rating 6 V 5 A max TS100LED MV TS100LED F MV White LED 13 Input ratings TS100 TS100 F Model for 100 110 and 120V AC areas Input voltage Select from 100 V 110 V or 120 V AC by relocating the fuse holder in the AC inlet Frequency 50 60 Hz Rated current 0 8 A or le...

Page 50: ...Use the above power supply cord only Using the wrong power cord may result in danger or fire The protection class I equipment should be connected to PE protective earth terminal TS100LED MV TS100LED F MV When used in 100 120 V region outside Japan UL listed detachable power cord set 3 conductor grounding 3 conductor grounding Type SVT No 18 AWG 3 m long maximum rated at 125 V AC minimum When used ...

Page 51: ...1 kg ECLIPSE TS100LED MV 228 W x 507 D x 480 H mm 8 5 kg ECLIPSE TS100LED F MV 228 W x 507 D x 480 H mm 9 0 kg 3 Operating Environmental Conditions Temperature 0 to 40 C Humidity 60 RH max no condensation Altitude 2 000 m max Pollution Degree 2 Installation category Category II Overvoltage category Electrical shock protection class Class I Indoor use only 4 Storage and Transport Environmental Cond...

Page 52: ...with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This product complies with Canadian EMI ICES 003 Class A This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numériq...

Page 53: ...目次 J 11 ...

Page 54: ... 3 2 2 3 1 4 4 1 0 4 4 5 5 6 5 0 7 Jp 䝬䞀䝱䝇䝕䛱䛐䛗䜑ᗣᲘ ืᅂ 䛴䜻䝷䝠䝯䝢䞀䜳䛙䛴䜻䝷䝠䝯䝢䞀䜳䛵ᮇ ဗ䛒 ื䛱ᅂ 䛛䜒䛰䛗䜒䛶䛰䜏䛰䛊䛙䛮䜘䛝䛬䛊䜄䛟䚯 ḗ㡧䛵ᮇ ဗ䜘䝬䞀䝱䝇䝕䟺㻨㻸䟻䛭䛟䜑ሔྙ䛱䛴䜅㐲䛛䜒䜄䛟䚯㻃 ᮇ ဗ䛵ᣞᏽ䛛䜒䛥㞗ሔᡜ䛭 ื䛱ᅂ 䛛䜒䜑䜎䛌䛱ᏽ䜇䜏䜒䛬䛊䜄䛟䚯 ᐓᗖ䜸䝣䛮䛝䛬ᗣᲘ䛝䛰䛊䛭䛕䛦䛛䛊䚯㻃リ䛱䛪䛊䛬䛵 ᗉ䜄䛥䛵ᆀᇡ䛴ᗣᲘ ฌ ᶭ㛭䛱䛚䛕䛦䛛䛊䚯 This symbol is provided for use in the People s Republic of China for environmental protection in the ﬁelds of electronic information products 䛙䛴䝢䞀䜳䛵䚮 ୯ᅗ䛴䛐ᐂᵕ䛱ྡྷ䛗䛥䜈䛴䛭䚮㞹Ꮔ ሒ ဗฦ...

Page 55: ...Amstelveen The Netherlands Laan van Kronenburg 2 1183 AS Amstelveen The Netherlands tel 31 20 44 96 300 tel 31 20 44 96 300 NIKON INSTRUMENTS SHANGHAI CO LTD NIKON INSTRUMENTS SHANGHAI CO LTD tel 86 21 6841 2050 tel 86 21 6841 2050 NIKON SINGAPORE PTE LTD NIKON SINGAPORE PTE LTD tel 65 6559 3618 tel 65 6559 3618 NIKON MALAYSIA SDN BHD NIKON MALAYSIA SDN BHD tel 60 3 7809 3688 tel 60 3 7809 3688 NI...

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