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Instruction

LSM700

2017

‐

07.docx

2017

‐

Confocal

LSM

700

Table

contents

Introduction

......................................................................................................................................

Hardware

..........................................................................................................................................

Objectives

......................................................................................................................................

Filter

turret

....................................................................................................................................

Laser

lines

......................................................................................................................................

Light

Path

......................................................................................................................................

Start

.............................................................................................................................................

Sample

mounting

and

viewing

.........................................................................................................

Software

...........................................................................................................................................

Sample

viewing

..............................................................................................................................

Acquisition

.....................................................................................................................................

LSM

Channel

mode

confocal

setting

.........................................................................................

Configuration:

........................................................................................................................

Acquisition

parameter

setting

............................................................................................

Single

image

acquisition

........................................................................................................

Multidimensional

acquisition

.................................................................................................

Shutting

down

.................................................................................................................................

Summary of Contents for LSM 700

Page 1: ...2 Objectives 2 Filter turret 2 Laser lines 3 Light Path 3 Start up 4 Sample mounting and viewing 5 Software 5 Sample viewing 6 Acquisition 8 LSM Channel mode confocal setting 8 Configuration 8 Acquis...

Page 2: ...pectral data point Hardware The microscope The LSM 700 is attached to an upright microscope Axio Imager Z2 Objectives Position Objective lens Magnification NA coverslip Working distance Immersion Parf...

Page 3: ...mission passes through the pinhole aperture and the out of focus light is eliminated 5 6 The variable secondary dichroic beamsplitter VSD is coated with a variable coating that between 420nm 630nm acc...

Page 4: ...ng order In the power strips turn on switches 1 2 Turn on the stage controller 3 Rotate clockwise the silver key in the lasers electronic unit 4 Turn on the computer and log in User name multilabs Pas...

Page 5: ...Light switch between the ocular and the confocal Via the touch pad 1 Choose the preferred objective 2 Choose the proper filter set and open the TL RL shutter Software Log into BookitLab and activate...

Page 6: ...tage to I II or III according to the objective lens see objective lens info on touchpad If you are acquiring transmitted channel set up Kohler illumination 1 Bring the sample into focus 2 Close the fi...

Page 7: ...p 7 2 5 3 4...

Page 8: ...ocal Configuration It is highly recommended to seek BCF staff s advice for new fluorophores exact name or new combinations Choose one Load a saved configuration from the Experiment Manager Reuse setti...

Page 9: ...ThermoFisher Chroma 1 Laser lines 2 Main dichroic mirror 3 PMTs PMT1 and PMT2 4 Variable secondary dichroic beamsplitter VSD 5 Emission Filter selection 6 Emission spectrum with Graphic representatio...

Page 10: ...logy experiments Frame size 512X512 Speed 8 Averaging Method mean in most cases in low signal with low noise Sum method may be useful Number 1 Mode frame for fixative sample for live cells and in case...

Page 11: ...lack level black blue pixels in the image o Set the PMT gain to 550 600 o Set the Laser it is recommended to use the lowest as possible and to increase gain master o In case of low signal with high la...

Page 12: ...ng to the SNR Press Snap in the start buttons Save the image press in the Images and Documents tab right hand side of the window and save in the directory D Users data PI name MM YYYY User name YYYY M...

Page 13: ...uch as brightness correction over z Time series In the Multidimensional Acquisition options check Time series a new tab Time Series will appear under the Multidimensional Acquisition Section Define nu...

Page 14: ...mended option the process can be configured in the processing main tool tab In the Acquisition mode tab Frame size Press optimal Zoom It is recommended to set the zoom above 1 2 Scanning speed 7 Avera...

Page 15: ...After a single scan in the Regions tab choose one of the ROI tools and draw on the acquired image the dimensions can also be set manually in the Width Height textboxes Define which process will be per...

Page 16: ...before moving to high magnification with small field of view Add a position by pressing Positions below the image container and clicking on the image container that was just acquired at the current st...

Page 17: ...lose the ZEN software Log off from your account in the BookItlab window Check if another user has a reservation if yes verify his her arrival If you are the last user for the day or the next reservati...

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Zeiss LSM 700, Instructions Manual

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