Zeiss LSM 700, Instructions Manual

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p. 6
Sample viewing
In the Locate tab choose the preferred objective filter set and open the illumination shutter
RL\TL

Set the Transmitted light (In case the TL is needed)
Click the transmitted light lamp icon, click ON and set desired brightness (or via the
microscope).
Set the filter turret position to DIC and the condenser below the stage to I, II or III according
to the objective lens (see objective lens info on touchpad).
If you are acquiring transmitted channel set up Kohler illumination:
1.
Bring the sample into focus.
2.
Close the field stop using the motorized aperture button (F) located at the right of the
microscope until you can see at least one edge
3.
Adjust the condenser height until the edges of the diaphragm image are crisp.
4.
Center the diaphragm image using the two centering screws.
5.
Open the field diaphragm, just until the image fills the field of view.