Promega Corporation ·
2800 Woods Hollow Road
·
Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526
·
Phone 608-274-4330
·
Fax 608-277-2516
· www.promega.com
Printed in USA.
Part# TM311
Revised 5/10
Page 37
11. Absorbance Photometer Operation
11.A. General Information
Four installed filters and two customizable filter holders are included on the
six-position filters wheel. The light source (LED) spectral range is 400–800nm.
Two absorbance wavelengths can be selected per well for running ratiometric
assays.
Microplate Recommendations
• Black-walled and clear flat-bottom plates are recommended for optimal
performance.
• Clear microplates may also be used.
Note:
When using clear microplates, use the Microplate Sample Tray Cover to
reduce light scatter from adjacent wells. See Section 20.D for removing and
attaching the Microplate Sample Tray Cover.
11.B. Optical Filters Wheel
The optical filters wheel (Figure 32) located on the bottom of the absorbance
detection head holds four fixed optical filters and two customizable filter
paddles. The wavelength is easily selected using the touch screen interface,
and the filter is selected automatically by the instrument. Manually changing
the filter is not required.
Figure 32. Optical Filters Wheel.
Diagram shows the locations of standard and
customizable filter paddles.
6999MB
600nm
“B” custom
“A” custom
750nm
450nm
560nm
