Promega E8031 Technical Manual Download Page 94

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Promega Corporation ·

2800 Woods Hollow Road

Madison, WI 53711-5399 USA

Toll Free in USA 800-356-9526

Phone 608-274-4330

Fax 608-277-2516

· www.promega.com

Printed in USA.

Part# TM311

Revised 5/10

Page 93

Luminescent Apoptosis Assays (continued)
Product

Cat.#

Equipment Required

Apo-ONE

Homogeneous Fluorescence

Module,

Caspase-3/7 Assay

G7792

Blue Optical Kit

Cell Signaling
Product

Cat.#

Equipment Required

cAMP-Glo™ Assay

V1501

Luminescence Module

PDE-Glo™ Phosphodiesterase Assay

V1361

Luminescence Module

CYP450 Screening Systems

Product

Cat.#

Equipment Required

P450-Glo™ CYP1A2 Screening System

V9770

Luminescence Module

P450-Glo™ CYP2C9 Screening System

V9790

Luminescence Module

P450-Glo™ CYP3A4 Screening System

V9800

Luminescence Module

P450-Glo™ CYP2C19 Screening System

V9880

Luminescence Module

P450-Glo™ CYP2D6 Screening System

V9890

Luminescence Module

ADME Assays
Product

Cat.#

Equipment Required

p450-Glo™ CYP3A4 Screening System
(Luciferin-PPXE) DMSO-Tolerant Assay

V9910

Luminescence Module

MAO-Glo™ Assay

V1401

Luminescence Module

Pgp-Glo™ Assay System

V3591

Luminescence Module

Kinase Assays
Product

Cat.#

Equipment Required

Kinase-Glo

Luminescent Kinase Assay

V6711

Luminescence Module

Kinase-Glo

Plus Luminescent

Kinase Assay

V3771

Luminescence Module

Protease Assays
Product

Cat.#

Equipment Required

DPPIV-Glo

™

Protease Assay*

G8350

Luminescence Module

Calpain-Glo™ Protease Assay

G8501

Luminescence Module

Proteasome-Glo™ 3-Substrate System*

G8531

Luminescence Module

*For Laboratory Use.

Summary of Contents for E8031

Page 1: ...T e c h n i c a l M a n u a l GloMax Multi Detection System INSTRUCTIONS FOR USE OF PRODUCTS E8031 E8041 E8051 E8061 E9031 E9061 PRINTED IN USA Revised 5 10 Part TM311...

Page 2: ...lti Detection System with Shaking 19 A Selecting Shaking Options 20 8 GloMax Multi Detection System with Temperature Control 21 A Activating the Temperature Control 21 B Setting Incubation Parameters...

Page 3: ...scence Optical Kits 45 14 Repeat Runs 46 A Repeat Runs Parameters 46 B Viewing Repeat Runs Data on the Results Screen 46 15 Data Management and Transfer 47 A Viewing the Data in the Results Screen 47...

Page 4: ...eaning the Injectors 78 F Cleaning the Waste Collection Tray 78 G Replacing Injector Tips 79 H Inserting Injector Tip Assembly 80 I Removing or Replacing Inlet and Outlet Plastic Tubing 80 J Removing...

Page 5: ...ard Light Plate optional G Non sterilized black or white 96 well plate H Waste Collection Tray included with injector system I Outlet Injector Tube Assembly included with injector system J DB 15 Seria...

Page 6: ...ts or physical damage due to accidentally bumping the open door Important Close the instrument door when the instrument is not in use The GloMax Multi Detection System is intended for indoor use only...

Page 7: ...ate Adapter 1 Protocol on CD Cat E9031 includes 1 GloMax Multi Base Instrument with Heating and Shaking 1 Power Cord 1 Power Supply Brick 24V 150W 1 USB Flash Drive 2GB 2 Optical Crosstalk Masks 1 Pla...

Page 8: ...lti Detection System Instrument Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 Phone 608 274 4330 Fax 608 277 2516 www promega com Printed in USA Pa...

Page 9: ...8 Revised 5 10 7026TA Home Screen 7027TA Help Screen 7028TA Opens and closes the instrument door Changes to a Stop button when a run is in progress Touch the Stop button to cancel a run if needed 702...

Page 10: ...036TA In Luminescence mode this button enables Kinetic Mode 7037TA In Absorbance mode the Wavelength button enables single or dual wavelengths 7038TA Starts a run 7039TA Activates the PMT before start...

Page 11: ...nce results the button toggles between the different data formats OD T and A 7048TA Toggles through the sets of results generated from repeat runs 7993TA Plate View button shows the full or partial vi...

Page 12: ...strument related information 7051TA Transfer instrument event log in txt format through the USB port to a USB flash drive 7053TA Sound on off 7054TA Set time and date 7055TA Port setting for PC Connec...

Page 13: ...ersion can be saved by touching the User button on the Protocol screen Prime The injector and tubing are rinsed and filled with the reagent Protocol Composer The section of the Graphical User Interfac...

Page 14: ...g the instrument Help Topics Covers informational topics From any of the Protocol wizards return to the Home screen by touching the Cancel button In the Instrument Control screen use the Home button f...

Page 15: ...eting the wizard you may save the protocol under the User list of protocols Select Protocol Allows you to retrieve stored protocols There are two types of stored protocols Preset and User The convenie...

Page 16: ...Setting Fluorescence Options screen Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 Phone 608 274 4330 Fax 608 277 2516 www promega com Printed in U...

Page 17: ...bel Make the desired change then touch the OK button 5 Touch the Plate button and select the wells to be read Green indicates that a well is selected 6 To run the modified protocol under the current n...

Page 18: ...ol Composer to Set Up Multiple Read Formats The Protocol Composer on the GloMax Multi System allows multiple assay requirements to be strung together into one protocol Simply select the parameters of...

Page 19: ...s selected this formatting will become the default for all subsequent selections Figure 11 Defining a multiple parameter protocol Figure 12 Defining user prompt message screen Promega Corporation 2800...

Page 20: ...allows one to define the parameters of shake operation such as time intensity and type of motion for the shaking pattern Time The GloMax Multi Detection System with Shaking allows for shaking interva...

Page 21: ...oser Figure 14 2 Touch the Time button to select the duration of shaking 3 Touch the Intensity button to select the rate of shaking desired 4 Touch the Type button to select the pattern of shaking des...

Page 22: ...tton on the Instrument Control Screen After two hours of inactivity the Temperature Control option will automatically shut off If the temperature of the instrument is different than the desired temper...

Page 23: ...ion 5 Touch the Temperature button to set the desired assay temperature in degrees Celsius C 6 Touch the Temperature Control button to activate the Heater controls 7 Touch the Door button to automatic...

Page 24: ...he Heater controls 6 Touch the Door button to automatically open the instrument door 7 Place the sample plate on the Microplate Sample Tray Well A1 must be at the top right corner 8 Touch the Door but...

Page 25: ...option to select either single or multiple readings per well The instrument reads from A1 to A12 B2 to B12 etc The read speed is similar in both the horizontal and vertical reading directions 1 Select...

Page 26: ...so toggles to Ref which refers to reference wells To select or deselect a whole row or column touch the corresponding number or letter Hold down the button and drag to select multiple rows or columns...

Page 27: ...e The detection mode needs to be set to Absorbance to enable this feature Touch a well to toggle between selected deselected and reference Figure 20 Reference wells must be selected individually It is...

Page 28: ...from the injector tip holder 2 Remove the injector tip holder from the optical head Facing the front of the GloMax Multi Detection System push the optical head upward then leftward Figure 21 Figure 2...

Page 29: ...head and gently guide the optical crosstalk mask onto the optical head Figure 23 Figure 23 Slide the 384 well optical crosstalk mask into place on the optical head 5 Return the injector tip holder to...

Page 30: ...g an average of those readings For 6 well plates the instrument will take a total of nine readings For 12 24 and 48 well plates the instrument will take five readings Figure 26 Viewing of all readings...

Page 31: ...also automatically open and close with the Microplate Sample Tray Do not use force to close the Microplate Sample Plate Cover Caution Do not use force to close the Microplate Sample Plate Cover The c...

Page 32: ...ample Tray Cover Flush the injectors after each use and clean up any spills See Section 20 for detailed cleaning instructions 10 B Microplate Sample Tray Cover The Microplate Sample Tray Cover is inst...

Page 33: ...tart button is touched before the PMT is completely activated the run will begin immediately after the 5 minute warm up of the PMT 10 D Starting a Run Easy to use protocol wizards can be accessed from...

Page 34: ...s file touch the Edit button to activate a keypad Enter the results file name and touch the OK button when editing is complete 11 Touch the OK button on the dialog screen to save The GloMax Multi Dete...

Page 35: ...ple plate to dark adapt The range is 0 60 minutes and can be adjusted in 1 minute increments Kinetic Interval The delay between the last reading of the previous well and the first reading or first inj...

Page 36: ...ght will turn Off once the Start button is released The light plate is now On and ready for use After five minutes it will automatically turn Off The built in timer can be restarted at any time by pre...

Page 37: ...d in USA Page 36 Revised 5 10 10 G Running the Standard Light Plate Protocol continued Figure 30 Viewing luminescence light plate results Figure 31 Calculating R2 value 7952TA 7951MA Luminescence Ligh...

Page 38: ...commended for optimal performance Clear microplates may also be used Note When using clear microplates use the Microplate Sample Tray Cover to reduce light scatter from adjacent wells See Section 20 D...

Page 39: ...Promega for the most current information on filter availability Wavelengths for custom filters cannot be entered by the user You have the option of selecting the custom positions labeled A or B To ass...

Page 40: ...icon from the Protocol Options to the Protocol Composer 3 Touch the white text button to select a protocol or modify the displayed parameters 4 Single or dual wavelengths can be selected by toggling t...

Page 41: ...the OK button on the dialog box The GloMax Multi Detection System will automatically begin reading samples 13 To interrupt a reading at any time touch the Stop button 14 Remove the sample plate after...

Page 42: ...nded Clear microplates can also be used For UV Vis region measurements UV transparent plates Costar Cat 3635 are recommended Note When using clear microplates we recommend using the Microplate Sample...

Page 43: ...filters come already installed for instruments ordered with the UV Vis Absorbance Module Cat E9061 The Absorbance Paddle can accommodate any 12 7mm in diameter and 6 4mm thick filter in the spectral r...

Page 44: ...strument includes a Luminescence Module it has a Microplate Sample Tray Cover installed The Microplate Sample Tray Cover is not required for using fluorescence mode To remove the Microplate Sample Tra...

Page 45: ...rag the Fluorescence icon from the Protocol Options to the Protocol Composer 3 Touch the Plate Format button and select the plate format that is required 4 Touch the Plate button and select the wells...

Page 46: ...t any time touch the Stop button 13 Remove the sample plate after the run is completed Note To prevent dehydration and spills remove the sample plate from the instrument when the reading is completed...

Page 47: ...l selected immediately after completing the last reading of the plate The actual period time is indicated on the results csv file when exported to a computer The range is between 1 to 120 minutes in 1...

Page 48: ...button is touched only the 50 most recent files are visible To view other results files touch the USB button to transfer all the files to a USB flash drive and view them on a computer 15 A Viewing th...

Page 49: ...results files 7993TA The results are displayed in a microplate format in either a full or partial view Toggle between the two views by touching the Plate View button between the horizontal arrows A p...

Page 50: ...taken for each sample Results are displayed side by side in the corresponding well partial view or first reading on top of the second reading full view To view a ratio of the two readings touch the R...

Page 51: ...d the results are displayed as a single value Figure 43 Panel C The ratio value calculated for absorbance data is different than for luminescence data The absorbance value is calculated as Absorbance...

Page 52: ...to open the file in Excel For Macintosh computers click to open the file and import the data into Excel Most USB flash drives are compatible with the GloMax Multi Detection System USB flash drives gr...

Page 53: ...uch screen is disabled 16 B System Requirements Windows based computer with Windows XP or Windows Vista operating system Microsoft NET Framework version 2 0 x86 Package is included with the External P...

Page 54: ...rivers for the GloMax Multi Detection System When connecting to a PC through a USB cable for the first time the computer will detect the instrument as a new device The drivers necessary to connect to...

Page 55: ...option Install the Software Automatically Select Next to continue 7077TA 7 The driver will begin to install A warning message will appear to inform the user that the driver has not passed Microsoft Wi...

Page 56: ...uter and confirm that the Instrument Port setting is on USB From this point all instrument control should be done from the computer To return control to the GloMax Multi Detection System follow the in...

Page 57: ...er It includes 2GB of space and has been tested to ensure compatibility with the GloMax Multi Detection System Most USB flash drives are compatible with the GloMax Multi Detection System USB flash dri...

Page 58: ...open 4 Slide the Fluorescence Detection Head into the position labeled Fluor located inside the instrument Be careful not to damage the printed circuit board on the back of the detection head 5 Hand t...

Page 59: ...purchasing and installation of the Absorbance Module See Section 22 D for ordering information Installing the Absorbance Housing Unit 1 Touch the Door button to automatically close the instrument door...

Page 60: ...and install the Visible Absorbance Module 8 Power on the instrument 9 To ensure that the instrument is detecting the newly installed Visible Absorbance Module verify that the Absorbance icon appears o...

Page 61: ...n head 6 Hand tighten the two captive screws using the Allen wrench size 7 16 inches provided with the kit Too much force may damage the screws See Figure 49 Figure 49 Arrows point to two captive scre...

Page 62: ...h the Door button to automatically close the instrument door and reset the Microplate Sample Tray to Home position 2 Power off the GloMax Multi Detection System and disconnect the power supply from th...

Page 63: ...pacer with the 1 5 inch Allen head screw using the provided 1 4 inch Allen wrench Figure 54 9 With all four extended instrument feet attached set the GloMax Multi Detection System back on its feet 10...

Page 64: ...ation and Operation 18 A Injector System Components Unpack the GloMax Multi Injector System carefully Verify all components and accessories have been received See the component list enclosed with the...

Page 65: ...ystem Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 Phone 608 274 4330 Fax 608 277 2516 www promega com Part TM311 Printed in USA Page 64 Revised 5...

Page 66: ...it down Insert the injector tip into the injector tip holder on the front of the Luminescence Module Note Injector tip 1 is on the left and tip 2 is on the right 7215TA Note During installation take c...

Page 67: ...p For dual injector systems ensure the injector tubing goes to the correct outer port Refer to the diagram located below the pumps for details 7017TA 11 Snap the stainless steel tube securely into the...

Page 68: ...ed with 900 l of the reagent to be injected Priming wets the fluid path and removes air bubbles to ensure delivery of an accurate injection volume for the assay Unused reagent in the fluid path can be...

Page 69: ...ectors are primed they are ready for use according to the programmed protocol Follow the Select Protocol wizard from the Home screen to select a saved protocol then go to the Instrument Control screen...

Page 70: ...eagent independent from a plate read This option can be used in conjunction with the Incubation option to inject reagent and then incubate the plate prior to a plate read The injector system delivers...

Page 71: ...sample plate into the instrument 9 Press the Start button 19 Tools From the Instrument Control screen touch the Tools button to manage various aspects of the instrument Figure 62 Promega Corporation 2...

Page 72: ...ation about the GloMax Multi Detection System The following product information is provided Part number and serial number of instrument Software version Part number and serial number of controller Fir...

Page 73: ...set to Pacific Standard Time The GloMax Multi Detection System does not automatically adjust for daylight savings time 19 E Updating Software The following instructions are used to update the GloMax M...

Page 74: ...ntrolling the GloMax Multi Detection System from an external PC For setup instructions see Section 16 Action Result 1 Copy the af ldf file to the top level of the USB flash drive 2 Insert the USB flas...

Page 75: ...Multi Detection System and disconnect the power supply whenever the interior of the instrument is open for cleaning and maintenance Residue accumulated from various reagents may inhibit proper moveme...

Page 76: ...adjustable 20 D Removing and Reinstalling the Microplate Sample Tray Cover Note The Microplate Sample Tray Cover should be used only with 96 well plates For all other plate sizes the cover must be rem...

Page 77: ...y assembly just in front of the crescent shaped tabs Figure 66 2 Gently push the Microplate Sample Tray Cover downward until a click is heard The click indicates the tray cover has been correctly plac...

Page 78: ...Microplate Sample Tray Cover the Break Away tab will break preventing damage to the tray assembly If the Break Away tab is broken the Tray Cover will not stay upright 1 Touch the Door button to autom...

Page 79: ...ent Control screen go to the Read screen Touch the Setup button and run the Flush protocol 10 Select the option for Custom Flush 11 Select nine flush cycles 12 When all flush cycles are complete empty...

Page 80: ...the injector tip assembly from the injector tip holder located on the Luminescence Module 3 Twist the white tip valve counterclockwise to release it from the gray tube fitting See Figure 68 for a pic...

Page 81: ...less Steel Tubing Replacement stainless steel tubing is available as part of the inlet tubing assembly Cat E8925 See Section 22 D 1 Hold the tan colored adapter just above the stainless steel tube 2 T...

Page 82: ...ding to the drawing 5 Reinstall the battery retainer plate 6 Replace the two Phillips head screws 7 Press the Start button while observing the Battery Check indicator to ensure the light plate is work...

Page 83: ...rument The instrument does not detect a microplate in the Microplate Sample Tray Make sure there is a microplate seated properly in the Microplate Sample Tray Protocol Selection No wells selected At l...

Page 84: ...y Injector 1 is installed correctly by checking all connections and looking for an Injector icon on the Instrument Status bar Injector 2 not installed The protocol requires addition of reagent using I...

Page 85: ...but the instrument is not detecting the presence of any Optical Kit Insert the desired Optical Kit into the detection head The color label of the Optical Kit should face up toward the user Firmly ins...

Page 86: ...agent residue has built up inside the injector injector tip holder tip holder Clean the holder with 70 ethanol and a damp cloth Injectors not injecting Air bubbles are blocking the line Flush the inje...

Page 87: ...t turned on low or blank before the run Touch the Start button on the Standard Light Plate It will automatically turn off after 5 minutes The selected wells do not correlate with the Standard Light Pl...

Page 88: ...Temperature 15 30 C 60 85 F Operating Humidity 5 75 noncondensing Warranty 1 year parts and labor Regulatory CE for research use only Luminescence Module Specifications Detector Head on photon countin...

Page 89: ...vailable UV Ex 365nm Em 410 460nm Blue Ex 490nm Em 510 570nm Green Ex 525nm Em 580 640nm Red Ex 625nm Em 660 720nm Custom kits upon request Detection Limit 0 5fmol 200 l or 1ppt of fluorescein in 96 w...

Page 90: ...se take the following steps 1 Contact Promega Technical Services 2 Carry out minor adjustments or tests as suggested by your Technical Services contact 3 If it is determined that the instrument should...

Page 91: ...irm that the returned items have not been used with body fluids or contaminated by toxic carcinogenic radioactive or other hazardous materials B I confirm that the returned items have been decontamina...

Page 92: ...h E5401 DB 15 Communication Cable 1 each E8081 Power Supply 24V 150W 1 each E8942 6 384 Well Plate Adapter 1 each E8943 96 Well Optical Crosstalk Mask 1 each E8944 384 Well Optical Crosstalk Mask 1 ea...

Page 93: ...inescence Module MultiTox Fluor Multiplex Fluorescence Module AFC Cytotoxicity Assay G9200 Optical Kit Blue Optical Kit MultiTox Glo Multiplex Fluorescence Module AFC Cytotoxicity Assay G9270 Optical...

Page 94: ...stem V9790 Luminescence Module P450 Glo CYP3A4 Screening System V9800 Luminescence Module P450 Glo CYP2C19 Screening System V9880 Luminescence Module P450 Glo CYP2D6 Screening System V9890 Luminescenc...

Page 95: ...Industries Ltd Costar is a registered trademark of Corning Inc Cy is a registered trademark of GE Healthcare Bio sciences Kimwipes is a trademark of Kimberly Clark Corporation LumiTrac is a trademark...

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