18.C. Injector System Operation (continued)
Priming Injectors
1. Place the reagent container into the reagent holder.
2. Cover the bottle opening with Parafilm
®
to prevent reagent evaporation.
3. Insert the stainless steel tube through the Parafilm
®
and into the reagent
bottle.
4. Snap the stainless steel tube into the slot on the horizontal bar, and ensure
the tube reaches the bottom of the reagent bottle.
5. Touch the “Door” button to automatically open the instrument door.
6. Place the Waste Collection Tray on the Microplate Sample Tray.
7. Touch the “Door” button to automatically close the instrument door.
8. From the Instrument Control screen, touch the “Read” button.
9. Touch the “Injector Setup” button (Figure 58). Prime the injector(s) by
following the step-by-step wizard.
10. After priming is complete, remove the Waste Collection Tray.
Figure 59. The Injector “Setup” button.
Once the injectors are primed, they are ready for use according to the
programmed protocol. Follow the Select Protocol wizard from the Home
screen to select a saved protocol, then go to the Instrument Control screen to
start the run.
Note:
Periodically inspect the injector tip(s) for bending or damage. Replace
them as needed.
Retrieving Unused Reagents from Injectors (optional)
1. From the Read screen, touch the “Injector Setup” button.
2. Follow the wizard to run Reverse Purge. Any reagents left in the fluid lines
will be returned to the reagent container. It may be necessary to run
Reverse Purge more than once to recover all unused reagent.
Flushing the Injectors after Use
1. Remove the sample plate from the instrument. Place the Waste Collection
Tray on the Microplate Sample Tray.
2. From the Instrument Control screen, touch the “Read” button, and then
touch the “Injector Setup” button.
Promega Corporation ·
2800 Woods Hollow Road
·
Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526
·
Phone 608-274-4330
·
Fax 608-277-2516
· www.promega.com
Part# TM311
Printed in USA.
Page 68
Revised 5/10
7087T
A
