10. Luminometer Operation
.................................................................................31
A. General Information...........................................................................................31
B. Microplate Sample Tray Cover ........................................................................31
C. PMT Activation...................................................................................................31
D. Starting a Run .....................................................................................................32
E. Kinetic Mode .......................................................................................................33
F. Kinetic Parameters .............................................................................................34
G. Running the Standard Light Plate Protocol ...................................................35
11. Visible Absorbance Photometer Operation
...............................................
37
A. General Information...........................................................................................37
B. Optical Filters Wheel..........................................................................................37
C. Starting a Run .....................................................................................................39
D. Ratiometric Assay...............................................................................................41
12. UV-Vis Absorbance Photometer Operation
...............................................41
A. General Information ..........................................................................................
41
B. Optical Filters Wheel .........................................................................................
41
C. Customizable Absorbance Paddles with 260nm and 280nm Filters ..........42
13. Fluorometer Operation
...................................................................................43
A. General Information ..........................................................................................
43
B. Optical Wavelengths and Commonly Used Fluorophores .........................
43
C. Starting a Run .....................................................................................................44
D. Switching Fluorescence Optical Kits ...............................................................45
14. Repeat Runs
.....................................................................................................46
A. Repeat Runs Parameters....................................................................................46
B. Viewing Repeat Runs Data on the “Results” Screen ....................................46
15. Data Management and Transfer
...................................................................47
A. Viewing the Data in the “Results” Screen......................................................47
B. Luminescence Data ............................................................................................49
C. Absorbance Data.................................................................................................49
D. Fluorescence Data...............................................................................................50
E. Multiplexing........................................................................................................50
F. Transferring Data ...............................................................................................51
16. Connecting to a PC
..........................................................................................52
A. General Information ..........................................................................................
52
B. System Requirements ........................................................................................
52
C. File Locations ......................................................................................................
52
D. Installing Microsoft .NET Framework version 2.0 (x86) ..............................53
E. Installing the Drivers for the GloMax
®
-Multi+ Detection System..............53
F. Connecting to a PC Using a USB Cable..........................................................55
G. Connecting to a PC Using an RS-232 Serial Cable ........................................55
H. Disconnecting from a PC...................................................................................56
I. USB Flash Drive..................................................................................................56
Promega Corporation ·
2800 Woods Hollow Road
·
Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526
·
Phone 608-274-4330
·
Fax 608-277-2516
· www.promega.com
Part# TM311
Printed in USA.
Page 2
Revised 5/10
