Promega Corporation ·
2800 Woods Hollow Road
·
Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526
·
Phone 608-274-4330
·
Fax 608-277-2516
· www.promega.com
Printed in USA.
Part# TM311
Revised 5/10
Page 35
10.G. Running the Standard Light Plate Protocol
The optional Standard Light Plate provides a quick way to verify instrument
performance. The Standard Light Plate consists of three highly stable light
sources that simulate luminescent samples at signal intensity ranging over
four logarithmic scales. Refer to the instruction card enclosed with the
Standard Light Plate for detailed instructions on use. The Standard Light Plate
protocol is available as a preset protocol.
Note:
See Section 15 for data analysis details.
Getting Started
1. Briefly press the “Start” button on the Microplate Luminescence Light
Plate.
2. The “Battery check” indicator will flash a green light as long as the “Start”
button is pressed and the battery has sufficient power.
3. The green light will turn Off once the “Start” button is released. The light
plate is now On and ready for use. After five minutes it will automatically
turn Off. The built-in timer can be restarted at any time by pressing the
“Start” button again.
4. If the green light does not appear while the “Start” button is pressed,
replace the battery. See Section 20.K for details.
Running the Luminescence Light Plate Protocol
5. From the Home Screen, choose Instrument Control.
6. From the Instrument Control screen, select Luminescence by pressing the
“Detection Mode” button.
7. After Luminescence has been selected, press the “Luminescence Protocol”
button and scroll down to Luminescence Light Plate.
8. Load the Luminescence Light Plate and press the “Start” button.
Analyzing the Results from the Luminescence Light Plate Protocol
9. Transfer the data file from the GloMax
®
-Multi+ Detection System to an
external computer via use of the USB flash drive.
10. Open the .csv file with Microsoft Excel and plot the results against 1 × 10
8
for B2, 1.2.× 10
6
for D2 and 1 × 10
4
for F2.
11. The R
2
value generated should be >0.98 (Figure 31). If the results are <0.98
contact Promega Technical Services for assistance.
